货号：09-809

品牌：Merck Millipore

规格：

目录价：￥4013.00

市场价格：￥3411.05

会员价格：￥3210.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-JMJD2A Antibody | 09-809 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~115 kDa observed View All» Immunogen: KLH-conjugated linear peptide corresponding to human JMJD2A. View All» Background Information: Jumonji domain-containing protein 2A (JMJD2A) is a nuclear and cytoplasmic member of the JMJD2 family, a group of proteins characterized as containing a JmjC domain, a JmjN domain, two PHD-type zinc finger domains, and 2 Tudor domains. Little is known about the function for most of these domains; however, they are believed to be involved in transcriptional regulation. JMJD2A functions as a histone demethylase with specific activity against trimethylated Histone H3 Lysine residues Lys-9 and Lys-36. It has no effect on any other lysine residues, or on mono- and dimethylated Lys-9 and Lys-36. The demethylation of these residues induces the production of succinate and formaldehyde. Studies have shown JMJD2A to recruit NCOR1 and histone deacetylases, causing the respective transcriptional repression of E2F- and ASCL2 responsive promoters. JMJD2A is ubiquitously expressed in human cell lines and tissues. View All» Species Reactivity:

• Human

• Mouse

• Chimpanzee

• Rhesus Macaque
  View All» Species Reactivity Note: Demonstrated to react with human and mouse. Predicted to react with chimpanzee and rhesus macaque based on 100% sequence homology. Other homologies: Rat (75% sequence homology). View All» Application Notes: Western Blot Analysis: 1 µg/mL from a previous lot detected JMJD2A in 10 µg of human testis tissue lysate.Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected JMJD2A in A431 cells.Chromatin Immunoprecipitation (ChIP) Analysis: A previous lot was used by an independent laboratory in ChIP on mouse embyronic cells (David Vetrie, University of Glasgow, Glasgow). View All» Control: U2OS nuclear extract View All» Quality Assurance: Evaluated by Western Blot in U2OS nuclear extract.Western Blot Analysis: 1 µg/mL of this antibody detected JMJD2A in 10 µg of U2OS nuclear extract. View All» Purification Method: Affinity Purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: O75164 View All» Entrez Gene Number: NP_055478.2 View All» Gene Symbol:
  • JHDM3A

  • JMJD2

  • JMJD2A

  • KDM4A
    View All» Alternate Names:
    • JMJD

    • lysine (K)-specific demethylase 4A

    • JmjC domain-containing histone demethylation protein 3A

    • jumonji domain containing 2A

    • jumonji C domain-containing histone demethylase 3A

    • jumonji domain containing 2

    • lysine-specific demethylase 4A

    • Jumonji domain-containing protein 2A
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry
        View All» Entrez Gene Summary: This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. COFACTOR: Binds 1 Fe2+ ion per subunit. SUBUNIT STRUCTURE: Interacts with histone deacetylase proteins HDAC1, HDAC2 and HDAC3. Interacts with RB and NCOR1. Interacts with HTLV-1 Tax protein. SUBCELLULAR LOCATION: Nucleus TISSUE SPECIFICITY: Ubiquitous. DOMAIN: The 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are able to bind trimethylated histone H3 'Lys-4', trimethylated histone H3 'Lys-9', di- and trimethylated H4 'Lys-20'. SEQUENCE SIMILARITIES: Belongs to the JHDM3 histone demethylase family.Contains 1 JmjC domain.Contains 1 JmjN domain.Contains 2 PHD-type zinc fingers.Contains 2 Tudor domains.SEQUENCE CAUTION: The sequence BAA31652.2 differs from that shown. Reason: Erroneous initiation. View All» Product Name: Anti-JMJD2A Antibody (09-809) View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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