货号：09-868

品牌：Merck Millipore

规格：200Y56

目录价：￥4621.00

市场价格：￥3927.85

会员价格：￥3696.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PHD finger protein 8 Antibody | 09-868 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~105 kDa observed. May recognize multiple isoforms. View All» Immunogen: KLH-conjugated linear peptide corresponding to human PHD finger protein 8. View All» Background Information: PHF8 (PHD finger protein 8) is a histone lysine demethylase. This protein preferentially demethylates histones that are monomethylated or dimethylated at lysine residues. Activity of this protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen. PHF8 is a member of the jumonji family and contains a jumonji C (JmjC) domain and a PHD (plant homeodomain) zinc-finger. Members of the jumonji protein family are known to play a role a chromatin remodeling and histone demethylation. Defects in PHF8 are the cause of mental retardation syndromic X-linked Siderius type. A disorder characterized by mild to borderline mental retardation with or without cleft lip/cleft palate. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Chimpanzee

• Bovine

• Rabbit

• Rhesus Macaque

• Canine
  View All» Species Reactivity Note: Demonstrated to react with Human and Mouse. Predicted to react with Rat, Chimpanzee, Bovine, Rabbit, Rhesus Monkey, Canine, and Porcine based on 100% sequence homology. View All» Application Notes: Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected PHD finger protein 8 in NIH/3T3, A431, and HeLa cells. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: 1:333 dilution of this antibody detected PHD finger protein 8 on 10 µg of HeLa cell lysate. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q9UPP1 View All» Entrez Gene Number: NP_001171825 View All» Gene Symbol:
  • PHF8

  • ZNF422
    View All» Alternate Names:
    • Histone lysine demethylase PHF8

    • PHD finger protein 8
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Immunoprecipitation
        View All» Entrez Gene Summary: The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3. CATALYTIC ACTIVITY: Protein N6,N(6)-dimethyl-L-lysine + 2-oxoglutarate + O2 = protein N(6)-methyl-L-lysine + succinate + formaldehyde + CO2. Protein N(6)-methyl-L-lysine + 2-oxoglutarate + O2 = protein L-lysine + succinate + formaldehyde + CO2. COFACTOR: Binds 1 Fe2+ ion per subunit Probable. SUBUNIT STRUCTURE: Interacts with POLR1B, UBTF, SETD1A, HCFC1, E2F1 and ZNF711. SUBCELLULAR LOCATION: Nucleus. Nucleus › nucleolus. Note: Recruited to H3K4me3 sites on chromatin during interphase. Dissociates from chromatin when cells enter mitosis. DOMAIN: The PHD-type zinc finger mediates the binding to H3K4me3. Binding to H3K4me3 promotes its access to H3K9me2. The linker region is a critical determinant of demethylase specificity. It enables the active site of JmjC to reach the target H3K9me2 when the PHD-type zinc finger binds to H3K4me3. PTM: Phosphorylation at Ser-69 and Ser-120 are required for dissociation from chromatin and accumulation of H4K20Me1 levels during prophase.INVOLVEMENT IN DISEASE: Defects in PHF8 are the cause of mental retardation syndromic X-linked Siderius type (MRXSSD) [MIM:300263]. A disorder characterized by mild to borderline mental retardation with or without cleft lip/cleft palate. SEQUENCE SIMLARITIES: Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.Contains 1 JmjC domain.Contains 1 PHD-type zinc finger.BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters:KM=134 µM for histone H3 H3K9Me2 Ref.23KM=8 µM for histone H3 H3K4me3 and H3K9Me2SEQUENCE CAUTION: The sequence BAA83063.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAB13877.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAI41577.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI41581.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI41582.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI42861.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI45929.1 differs from that shown. Reason: Erroneous termination at position 419. Translated as Arg. View All» Product Name: Anti-PHD finger protein 8 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-136905.htm
        © Merck Millipore代理09-868 Anti-PHD finger protein 8 Antibody;store at +2℃ to +8℃，产品报价联系微信jinshanbio