金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived Accession #:Q62997 N-terminal Sequence Analysis:Asp25 Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Predicted Molecular Mass:74 kDa (monomer) SDS-PAGE:95 kDa, reducing conditions Activity:Measured by its ability to confer GDNF responsiveness to PC‑12 rat adrenal pheochromocytoma cells using a neurite outgrowth assay. Treanor, J. et al. (1996) Nature 392:80. The ED₅₀ for this effect is approximately 0.3 µg/mL in the presence of 25ng/mL of Recombinant Rat GDNF (Catalog# 512-GF).Measured by its binding ability in a functional ELISA.Immobilized Recombinant Rat GDNF (Catalog# 512-GF) at 1 µg/mL binds Recombinant Rat GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera with an apparent K_D <5nM. Molecule Information: GFR alpha-1/GDNF R alpha-1 Long Name: Glial Cell line-derived Neurotrophic Factor Receptor alpha 1 Aliases: GDNF R alpha-1; GFRA1 Entrez Gene IDs: 2674 (Human); 25454 (Rat) Background: GFR alpha View GFR alpha IHC images. GFR alpha -1, -2, -3, and -4 are GPI-anchored receptors that interact preferentially with the GDNF family ligands GDNF, Neurturin, Artemin, and Persephin, respectively. GFR alpha proteins initiate signaling in association with the receptor tyrosine kinase RET and by RET-independent pathways. GFR alpha-mediated signaling is instrumental in the differentiation, migration, and support of central and peripheral neurons. Alternative splicing generates multiple isoforms of GFR alpha proteins.