金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Lys2-Lys619 with an N-terminal Met and 6-His tag Accession #:AAM55479 N-terminal Sequence Analysis:Met Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:75 kDa SDS-PAGE:65 kDa, reducing conditions Activity:Measured by its ability to cleave alpha -N-acetylgalactosaminyl from 4-Nitrophenyl N-acetyl-alpha -D-galactosaminide.The specific activity is >5,000 pmol/min/ug, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris and NaCl.See Certificate of Analysis for details. Molecule Information: alpha-N-acetylgalactosaminidase/NAGA Aliases: NAGA Entrez Gene IDs: 4668 (Human); 17939 (Mouse); 315165 (Rat) Background: alpha-N-acetylgalactosaminidase The ABO blood group system is the most important blood type system and alpha-N-acetylgalactoside is key to the ABO blood group antigen. alpha-N-acetylgalactosidase from Clostridium perfringens is a useful tool for removing alpha linked N-acetylgalactosamine from blood type A antigen to produce H antigen and blood type O. Blood type O is universally compatible in the ABO system and is widely used in blood transfusion. The enzyme was highly selective for terminal N-acetylgalactosamine residues. No other exoglycosidase activities, particularly neuraminidase, was detected. Recombinant alpha-N-acetylgalactosidase from Clostridium perfringens can potentially be used in enzymatic conversion of human blood type A red blood cells to universally transfusable type O red blood cells.