金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived, Cys24-Ala217 Accession #:P30120 N-terminal Sequence Analysis:Cys24 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:21.5 kDa SDS-PAGE:32-34 kDa, reducing conditions Activity:Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH₂ (Catalog# ES001).The IC₅₀ value is <3.0nM, as measured under the described conditions. Formulation:Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.See Certificate of Analysis for details. Molecule Information: TIMP-1 Long Name: Tissue Inhibitors of Metalloproteinases 1 Entrez Gene IDs: 7076 (Human); 21857 (Mouse); 116510 (Rat) Background: TIMPs TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.