货号：12-414

品牌：Merck Millipore

规格：200Ug

目录价：￥3070.00

市场价格：￥2609.50

会员价格：￥2456.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: CHKtide, biotin conjugate View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 200 µg View All» Key Applications: Kinase Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» UniProt Summary: FUNCTION: SwissProt: O14757 # Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X- S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser- 293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK- cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.SIZE: 476 amino acids; 54420 Da SUBUNIT: Interacts with BRCA1, CLSPN, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta.SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.TISSUE SPECIFICITY: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.DOMAIN: SwissProt: O14757 The autoinhibitory region (AIR) inhibits the activity of the kinase domain.PTM: Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from checkpoint mediated cell cycle arrest. May also be phosphorylated at Ser-280 by AKT1/PKB, which may promote mono and/or diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, which results in decreased activity. & Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion (By similarity).SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily. & Contains 1 protein kinase domain. View All» Quality Assurance: Routinely evaluated as a substrate for CHK enzymes. View All» Brand Family: Upstate View All» Presentation: sterile, distilled water View All» Protein Target: CHK View All» UniProt Number: O14757 View All» Target Sub-Family: CAMK View All» Storage Conditions: 1 year at -20°C View All» Gene Symbol:

• CHEK1

• CHK1
  View All» Product Name: CHKtide, biotin conjugate View All» Entrez Gene Number: NM_001274.3 View All»
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