金山科研平台，产品价格货期咨询微信：jinshanbio Source:Spodoptera frugiperda, Sf 21 (baculovirus)-derived, Met1-Thr279, with a C-terminal 6-His tag Accession #:Q9HAN9 N-terminal Sequence Analysis:Ser4 Structure:Non-covalently linked hexamer Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:32 kDa SDS-PAGE:33 kDa, reducing conditions Activity:Measured by the production of NAD⁺, which is converted to NADH by alcohol dehydrogenase.The specific activity is >2,500 pmol/min/µg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris, NaCl, Glycerol and DTT.See Certificate of Analysis for details. Molecule Information: NMNAT-1 Long Name: Nicotinamide mononucleotide adenylyltransferase-1 Aliases: NMN adenylyltransferase 1; PNAT1 Entrez Gene IDs: 64802 (Human) Background: NMNAT-1 NMNAT-1 is expressed in the nuclei of all human tissues, with highest expression in skeletal muscle, heart, kidney, pancreas, and brain. The enzyme transfers adenylate from ATP to nicotinamide ribonucleotide or nicotinate ribonucleotide to generate NAD+ or deamido-NAD+, and is an essential enzyme for the production of nuclear NAD+. Nuclear NAD+ is required by poly(ADP-ribose) polymerase 1 (PARP-1), which poly-ADP-ribosylates chromatin in response to DNA strand breaks. NMNAT-1 is known to interact with PARP-1, resulting in its activation, but this interaction with PARP-1 is prevented when NMNAT-1 is phosphorylated at Ser136. Nuclear NAD+ levels are also important for the regulation of SIR2 histone deacetylases. A naturally occurring Ube4b/NMNAT-1 chimeric protein is directly involved in slowing the degeneration of injured neurons in mice. NMNAT activity is required for the activation of tiazofurin, a drug used to treat leukemia. Two other NMNAT enzymes are present in humans: NMNAT-2 is localized in the Golgi complex and cytoplasm, and NMNAT-3 is a mitochondrial enzyme.