金山科研平台，产品价格货期咨询微信：jinshanbio Source:Spodoptera frugiperda, Sf 21 (baculovirus)-derived, Phe93-Ser201, with an N-terminal Met and C-terminal 6-His tag Accession #:O43915 N-terminal Sequence Analysis:Met Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:13 kDa (monomer) SDS-PAGE:17-20 kDa, reducing conditions Activity:Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. Marconcini, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9671. The ED₅₀ for this effect is typically 0.1‑0.3µg/mL.Measured by its binding ability in a functional ELISA.Immobilized recombinant human Flt-4 Fc Chimera at5 µg/mL (100 µL/well) binds Recombinant Human VEGF‑D with an apparent K_D <15 nM. Molecule Information: VEGF-D Long Name: Vascular Endothelial Growth Factor D/cFos-induced Growth Factor Aliases: FIGF Entrez Gene IDs: 2277 (Human); 14205 (Mouse) Background: VEGF-D View VEGF-D IHC images.

Vascular endothelia growth factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are characterized by eight conserved cysteine residues that form a cystine knot structure. VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and C-terminal propeptide extensions around the VEGF homology domain (VHD). Proteolytic processing of the 354 aa VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of non-covalently linked 42 kDa homodimers of the 117 aa VHD.

Mature human VEGF-D shares 94%, 95%, 99%, 97% and 93% aa identity with mouse, rat, equine, canine and bovine VEGF-D, respectively. It is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin. Mouse and human VEGF-D are ligands for VEGF receptor 3 (VEGF R3, also called Flt-4) that are active across species and show enhanced affinity when processed. Processed human VEGF-D is also a ligand for VEGF R2, also called Flk-1 or KDR. VEGF R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium. While VEGF-C is the critical ligand for VEGF R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth. Both promote tumor lymphangiogenesis. Consonant with their activity on VEGF receptors, binding of VEGF-C and VEGF-D to neuropilins contributes to VEGF R3 signaling in lymphangiogenesis, while binding to integrin α9β1 mediates endothelial cell adhesion and migration.