金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Met1-Asn317, with a C-terminal 6-His tag Accession #:P08721 N-terminal Sequence Analysis:Leu17 Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:34.1 kDa SDS-PAGE:60-65 kDa, reducing conditions Activity:Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.

When1 x 10⁵ cells/well are added to Recombinant Rat Osteopontin/OPN coated plates, cell adhesion is enhanced in a dose dependent manner after 1hour incubation at 37 °C. The ED₅₀ for this effect is typically 0.2 -1 µg/mL.