金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Gln38-Lys380 Accession #:P22725 N-terminal Sequence Analysis:No results obtained: Gln38 predicted Purity:>80%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:38 kDa SDS-PAGE:45 kDa, reducing conditions Activity:Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.The ED₅₀ for this effect is typically 0.1 - 0.5 µg/mL, in the presence of5ng/mL rmWnt-3a. Optimal concentrations should be determined by each laboratory for each application. Molecule Information: Wnt-5a Long Name: Wingless-type MMTV Integration Site Family, Member 5a Entrez Gene IDs: 7474 (Human); 22418 (Mouse) Background: Wnt-5a View Wnt-5a IHC images.

Wnt proteins are secreted glycoproteins that play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca²⁺ pathway, playing roles in cell movements and cell adhesion. This non-canonical Wnt pathway can inhibit canonical Wnt/beta-catenin signaling. In Wnt-5a deficient mouse embryos, beta-catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta-catenin. Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis.

Wnt-5a is implicated in various types of cancer and has complex roles. Wnt-5a is known to act as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells. In contrast, Wnt-5a is upregulated in melanomas, where expression levels correlate with severity of metastasis. Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis. Like other developmental growth factors Wnt-5a has diverse roles in development which range from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mouse and human Wnt-5a share 97% amino acid identity.