金山科研平台，产品价格货期咨询微信：jinshanbio Source:Spodoptera frugiperda, Sf 21 (baculovirus)-derived, Met1-Asn481, with a C-terminal 10-His tag. Accession #:O88947 N-terminal Sequence Analysis:Tyr84, Ile232 Structure:Recombinant Mouse Coagulation Factor X is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks theHis tag. Purity:>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane. Endotoxin Level: Predicted Molecular Mass:29 kDa & 10 kDa SDS-PAGE:30-38 kDa & 13-15 kDa, reducing conditions Activity:Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH₂ (Catalog# ES002).The specific activity is >300 pmol/min/µg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in MES, NaCl and CaCl₂.See Certificate of Analysis for details. Molecule Information: Coagulation Factor X Aliases: Cf10; F10; FX; FXa; Prothrombinase; Stuart Factor Entrez Gene IDs: 2159 (Human); 14058 (Mouse); 29243 (Rat) Background: Coagulation Factor X Coagulation Factor X is a vitamin K-dependent plasma protease that activates thrombin in blood coagulation. Initially synthesized in the liver as a single-chain precursor, factor X is activated by both the intrinsic and extrinsic pathways to Xa, which consists of disulfide bond-linked light and heavy chains. The light chain contains a Gla and two EGF-like domains and the heavy chain corresponds to the serine protease domain.