金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived, Met1-Asn707, with a C-terminal 6-His tag Accession #:AAH04637 N-terminal Sequence Analysis:Ser17 and Ile463 Purity:>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane. Endotoxin Level: Predicted Molecular Mass:51kDa (heavy chain) & 28 kDa (light chain) SDS-PAGE:58-59 kDaand 35-36 kDa, reducing conditions Activity:Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.The specific activity is >5,500 pmol/min/µg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris, CaCl, NaCl, and Glycerol.See Certificate of Analysis for details. Molecule Information: Complement Component C1ra Aliases: C1ra Entrez Gene IDs: 50909 (Mouse) Background: Complement Components/Factors Nine serine proteases and their cofactors participate in the three pathways that lead to complement activation. C1r and C1s are the first two proteases that trigger the classical pathway. Three mannan-binding lectin-associated serine proteases (MASPs) are involved in the MBL pathway. C2 is involved in both the classical and the MBL pathways. Factor D is the first protease that activates the alternative pathway, which also includes proteases factor B and factor I. C3 plays a central role in both classical and alternative pathways. C3d, one of its fragments, binds to CR2. C5 is common to all three pathways, and C5a belongs to a family of related proteins known as anaphylatoxins.