金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Ala22-His314, with an N-terminal HA (YPYDVPDYA) tag Accession #:NP_061250 N-terminal Sequence Analysis:Tyr (N-terminus of Hemagglutinin tag) Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Predicted Molecular Mass:34.3 kDa SDS-PAGE:39-42 kDa, reducing conditions Activity:Measured by its ability to inhibit activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.The ED₅₀ for this effect is typically 0.1 -0.5 µg/mL in the presence of 50 ng/mL Recombinant Mouse Wnt-3a (Catalog# 1324-WN). Formulation:Lyophilized from a 0.2 µm filtered solution in PBS and NaCl.See Certificate of Analysis for details. Molecule Information: sFRP-5 Long Name: Secreted Frizzled Related Protein 5 Aliases: FRP1B; SARP3 Entrez Gene IDs: 6425 (Human) Background: Secreted Frizzled Related Proteins (sFRP) Secreted Frizzled related proteins are secreted glycoproteins with homology to the Wnt-binding domain of the Frizzled family of transmembrane receptors. sFRPs contain an N-terminal Frizzled-like cysteine-rich domain (CRD) with 10 conserved cysteines and a C-terminal heparin-binding region. sFRPs function as soluble antagonists of Wnt signaling by binding directly to Wnt proteins and preventing their interactions with Frizzled receptors.