金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Ser2-Arg212, with a C-terminal 6-His tag Accession #:Q64373 N-terminal Sequence Analysis:Ser2 Structure:Monomer and disulfide-linked homodimer Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Predicted Molecular Mass:25 kDa SDS-PAGE:27 kDa, under reducing and non-reducing conditions Activity:Measured by its ability to inhibit Caspase-8-cleaved BID-mediated release of cytochromec from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA (Catalog# MCTC0) to quantify cytochromec.The typical EC₅₀ for Bcl-x_L in this assay is <400nM in the presence 54nM of Recombinant Human or Mouse BID Caspase-8-cleaved (Catalog# 882-B8 or 883-M8, respectively). Optimal dilutions should be determined by each laboratory for each application. Formulation:Supplied as a 0.2 µm filtered solution in HEPES and KCl.See Certificate of Analysis for details. Molecule Information: Bcl-xL Long Name: B Cell Lymphoma/Leukemia x Long Form Entrez Gene IDs: 598 (Human); 12048 (Mouse); 24888 (Rat) Background: Bcl-x Bcl-x is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Alternative splicing results in two distinct Bcl-x isoforms. Bcl-xL (long) is an anti-apoptotic protein that prevents release of Cytochromec from the mitochondrial intermembrane space into the cytosol. Bcl-xs (short) is a pro-apoptotic member that can initiate programmed cell death.