金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Met1-Asp195, protein was purified, cleaved by Caspase-8 and further purified Accession #:P55957 N-terminal Sequence Analysis:Met1 (N-terminal fragment) & Gly61 (C-terminal fragment) Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Predicted Molecular Mass:7 kDa (N-terminal fragment) & 15 kDa (C-terminal fragment) SDS-PAGE:9kDa &14 kDa,reducing conditions Activity:Measured by its ability to induce cytochromec release from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA (Catalog# MCTC0) to quantify cytochromec.The typical EC₅₀ for cytochromec releasing activity is <50nM.The EC₅₀ for the desired application should be determined. Uncleaved Recombinant Human BID (Catalog# 846-BD) is available. Formulation:Supplied as a 0.2 µm filtered solution in HEPES-KOH and KCl.See Certificate of Analysis for details. Molecule Information: BID Long Name: BH3 Interacting Domain Death Agonist Entrez Gene IDs: 637 (Human); 12122 (Mouse) Background: BID (BH3 Interacting Domain Death Antagonist) BID is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. BID is a proapoptotic member that causes Cytochrome c to be released from the mitochondrial intermembrane space into the cytosol. In healthy cells, BID is cytosolic. In response to Fas Ligand or TNF-alpha, BID is cleaved by Caspase-8 and then relocates to the mitochondrial outer membrane.