金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived, Ala18-Ile303, with a C-terminal 10-His tag Accession #:NP_003109 N-terminal Sequence Analysis:Ala18 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:34 kDa SDS-PAGE:40-50 kDa, reducing conditions Activity:Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. Schiemann,B.J. etal. (2003) Mol. Biol. Cell. 14:3977. The ED₅₀ for this effect is typically 0.75 - 3.0 µg/mL.Measured by its binding ability in a functional ELISA.Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind rhSPARC with an apparent K_D < 40 nm. Optimal dilutions should be determined by each laboratory for each application. Formulation:Lyophilized from a 0.2 µm filtered solution in PBS.See Certificate of Analysis for details. Molecule Information: SPARC Long Name: Secreted Protein Acidic and Rich in Cysteine Aliases: BM-40; Osteonectin Entrez Gene IDs: 6678 (Human); 20692 (Mouse) Background: SPARC SPARC, also known as Osteonectin and Basement Membrane protein BM-40, is a phosphorylated glycoprotein with calcium-binding domains. It is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC may be involved in cell proliferation, repair of tissue damage, and morphogenic processes such as re-modeling of the extracellular matrix.