货号：14-601M

品牌：Merck Millipore

规格：250Ug

目录价：￥41939.00

市场价格：￥35648.15

会员价格：￥33551.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ROKβ/ROCK-I, active View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 250 µg View All» Product Overview: N-terminal His6-tagged, recombinant, human ROCK-I amino acids 17-535; autoactivated by incubating with Mg/ATP and redialysed to remove excess ATP View All» Key Applications: Kinase Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. View All» UniProt Summary: FUNCTION: SwissProt: Q13464 # Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Necessary for apoptotic membrane blebbing. Plays a role in smooth muscle contraction. Required for centromere positioning and centromere- dependent exit from mitosis (By similarity).SIZE: 1354 amino acids; 158175 Da SUBUNIT: Binds RHOA that has been activated by GTP binding. Binds ADD1, GEM, RHOB, RHOC, myosin light chain and VIM (By similarity). Binds RHOE, PPP1R12A (MYPT1/MBS), LIMK1 and LIMK2.SUBCELLULAR LOCATION: Cytoplasm. Centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes.TISSUE SPECIFICITY: Detected in blood platelets.DOMAIN:SwissProt: Q13464 The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.PTM: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR. & Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 REM (Hr1) repeat. MISCELLANEOUS: Inhibited by Y-27632. View All» Species: Human View All» Molecular Weight: 61.4kDa View All» Brand Family: Upstate View All» Source: expressed by baculovirus in Sf21 insect cells View All» Protein Target: ROKβ/ROCK-I View All» Storage Conditions: 6 months at -70°C View All» Target Sub-Family: AGC View All» Promotional Text: This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service. View All» Gene Symbol:

• ROCK1

• P160ROCK

• MGC131603

• p160ROCK

• MGC43611

• p160-ROCK
  View All» Product Name: ROKβ/ROCK-I, active View All» Quality Assurance: routinely evaluated by phosphorylation of Long S6 substrate peptide (Catalog #12-420) View All» UniProt Number: Q13464 View All» Specific Activity: For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme View All» Packaging: Also available in 10 μg size (cat#14-601) and in bulk (cat# 14-601-K). View All» Entrez Gene Number: NM_005406.2 View All» Purification Method: Ni²⁺/NTA-agarose View All»
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  © Merck Millipore代理14-601M ROKβ/ROCK-I Protein, active, 10 µg;ROKβ/ROCK-I蛋白，活性，10 µgstore at -80℃，产品报价联系微信jinshanbio