金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Asn971-Arg1023, with an N-terminal Met Accession #:P01133 N-terminal Sequence Analysis:Met Purity:>97%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level:< 0.10 EU per 1 μg of the protein by the LAL method. Predicted Molecular Mass:6 kDa Activity:Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415.The ED₅₀ for this effect is typically 20-100 pg/mL. Formulation:Lyophilized from a 0.2 µm filtered solution in PBS.See Certificate of Analysis for details. Molecule Information: EGF Long Name: Epidermal Growth Factor Aliases: HOMG4; URG; Urogastrone Entrez Gene IDs: 1950 (Human); 13645 (Mouse); 25313 (Rat) Background: EGF View EGF IHC images.

Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands. Its actions were shown to induce early eyelid opening, incisor eruption, hair growth inhibition, and stunt growth when injected into newborn mice. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone, accordingly. EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active.

A wide variety of in vitro and in vivo biological effects have been ascribed to EGF and other members of the EGF family. The best documented activity of EGF is its ability to promote proliferation and differentiation of mesenchymal and epithelial cells. In vitro, EGF is a mitogen for fibroblasts, epithelial and endothelial cells, and promotes colony formation of epidermal cells in culture. In vivo, EGF induces epithelial development, promotes angiogenesis, and inhibits gastric acid secretion.