货号：14-737

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: MEK1 (K97R), inactive View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 50 µg View All» Product Overview: Recombinant full length human MEK1 containing a Lysine to Arginine mutation at position 97 and a C-terminal 6His-tag. MEK1 (K97R) is a suitable substrate for Raf-1, but is not activated upon phosphorylation. View All» Key Applications: Enzyme Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. View All» UniProt Summary: FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.SIZE: 393 amino acids; 43439 Da SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.DISEASE: SwissProt: Q02750 # Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.SIMILARITY: SwissProt: Q02750 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain. View All» Species: Human View All» Molecular Weight: 45 kDa View All» Brand Family: Upstate View All» Source: expressed in E. coli View All» Protein Target: MEK1 View All» Presentation: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 10% glycerol, 0.1% 2-mercaptoethanol, and 0.2mM PMSF. View All» Storage Conditions: Stable for 6 months at -70°C from date of shipment. View All» Target Sub-Family: STE View All» Gene Symbol:

• MAP2K1

• PRKMK1

• MKK1

• MAPKK1

• MEK1
  View All» Product Name: MEK1 (K97R), inactive View All» Alternate Names: MAPKK1; ERK activator kinase 1; MAPK/ERK kinase 1 View All» Quality Assurance: MEK1 (K97R), inactive is a suitable substrate for Raf-1, but is not activated upon phosphorylation. Raf-1 phosphorylation and the absence of MEK1 activity towards downstream kinases was assessed by immunoblot analysis using anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) (Catalog # 07-461) and anti-phospho-MAP Kinase1/2 (Erk1/2) (Catalog # 07-467). View All» UniProt Number: Q02750 View All» Specific Activity: For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. View All» Entrez Gene Number: NM_002755.2 View All» Purity: >80% by SDS PAGE and Coomassie blue staining. View All» Purification Method: Ni²⁺/NTA-agarose View All»
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