货号：14-836

品牌：Merck Millipore

规格：

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ACF complex (Acf1/ISWI), active View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 10 μg View All» Product Overview: Drosophila ACF complex is prepared by coexpression of C-terminal FLAG-tagged Acf1 subunit with untagged ISWI subunit by baculovirus in Sf9 cells. View All» Background Information: Acf1 and ISWI are subunits of CHRAC (chromatin accessibility complex). ACF1-ISWI chromatin-remodeling complex is required for replication through heterochromatin in mammalian cells. View All» Molecular Weight: Acf1 MW = 185 kDa, ISWI MW = 140 kDa. View All» Quality Assurance: Activity was routinely tested with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410). View All» Brand Family: Upstate View All» Source: Coexpression of C-terminal FLAG-tagged Acf1 subunit with untagged ISWI subunit by baculovirus in Sf9 cells View All» Presentation: 50 mM Tris-HCl pH8.0, 150 mM NaCl, 2 mM MgCl2, 0.2 mM EDTA, 10% (v/v) glycerol, 500 μg/ml FLAG peptide. Frozen solution. View All» Format: Purified View All» UniProt Number: ISWI: Q24368, Acf1: Q9V9T4 View All» Specific Activity: Varies with lot. Lot # R0803D0003: 0.5 µg ACF complex has been successfully used for 1 chromatin assembly reaction with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410). View All» Storage Conditions: Stable for 6 months at -80°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap. View All» Product Name: ACF complex (Acf1/ISWI), active View All» Entrez Gene Number: Acf1: 43751; ISWI: 36390 View All» Purity: Varies with individual lot. Lot R0803D0003 has purity ~80% by SDS-PAGE and Coomassie blue staining View All» Purification Method: Purified by FLAG immunoaffinity chromatography View All»