货号：14-851

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: UbcH12 Conjugating Enzyme View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 100 µg View All» Product Overview: The enzyme was expressed in E. coli and purified using Ni²⁺ chelation chromatography with Ni²⁺ in limiting concentration. This preparation is functional in in vitro NEDDylation reactions. UbcH12 has been shown to form a thioester linkage with NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. A dominant negative form of UbcH12, previously demonstrated to sequester NEDD8 and inhibit its conjugation, inhibits both conjugation and processing of p105, which is alleviated by wild-type UbcH12. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. View All» UniProt Summary: FUNCTION: SwissProt: P61081 # Accepts the ubiquitin-like protein NEDD8 from the UBE1C- APPBP1 E1 complex and catalyzes its covalent attachment to other proteins. Involved in cell proliferation.SIZE: 183 amino acids; 20900 Da SUBUNIT: Interacts with UBE1C.DOMAIN: SwissProt: P61081 Both the N-terminal docking peptide and the catalytic core domain must bind the UBE1C-APPBP1 complex simultaneously for optimal transfer of NEDD8.SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily. View All» Species: Human View All» Molecular Weight: 22 kDa View All» Brand Family: Upstate View All» Source: Recombinant human UbcH12 Conjugating Enzyme expressed in E. coli View All» Presentation: 100 μg of purified protein at a concentration of 0.5 mg/mL in 20 mM Tris-HCl, pH 7.5, containing 0.5 mM DTT. View All» Storage Conditions: As supplied, store at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. After initial defrost, aliquot (for extended storage it is recommended that aliquots be no smaller then 10 μL) and store at -80°C. Snap-freezing in a dry ice/ethanol bath or liquid nitrogen is recommended. As supplied, the enzyme is stable on ice for several hours. View All» Gene Symbol:

• UBE2M

• hUbc12

• UBC12

• UBC-RS2
  View All» Product Name: UbcH12 Conjugating Enzyme View All» UniProt Number: P61081 View All» Entrez Gene Number: NM_003969.3 View All» Purity: >95% by SDS-PAGE View All»
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