货号：14-852

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: UbcH13/Mms2 Conjugating Enzyme View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 100 µg View All» Product Overview: The enzyme complex was co-expressed in E. coli and purified using Ni2+ chelation chromatography with Ni2+ in limiting concentration. A heterodimer composed of the catalytically active ubiquitin-conjugating enzyme UbcH13 and its catalytically inactive paralog hMms2, which lacks an active site cysteine residue. The UbcH13/Mms2 complex catalyzes synthesis of Lys-linked, rather than Lys-linked, ubiquitin chains, which serve as non-proteolytic tags involved in error-free post-replicative DNA repair and NF-κB signal transduction. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: Ubiquitin-conjugating enzyme E2 variant proteins constitute a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. The protein encoded by this gene also shares homology with ubiquitin-conjugating enzyme E2 variant 1 and yeast MMS2 gene product. It may be involved in the differentiation of monocytes and enterocytes. View All» UniProt Summary: FUNCTION: SwissProt: Q15819 # Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-62'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.SIZE: 145 amino acids; 16363 Da SUBUNIT: Heterodimer with UBE2N. Binds CHFR.TISSUE SPECIFICITY: Detected in placenta, colon, liver and skin. Detected at very low levels in most tissues.SIMILARITY: SwissProt: Q15819 ## Belongs to the ubiquitin-conjugating enzyme family. View All» Species: Human View All» Molecular Weight: 18.4 kDa and 16.4 kDa View All» Brand Family: Upstate View All» Source: Recombinant human UbcH13/Mms2 Conjugating Enzyme expressed in E. coli View All» Presentation: 100 μg of protein at a concentration of 1.0mg/mL in 50 mM HEPES, pH 8.0, 50 mM NaCl, 10% Glycerol, 1 mM DTT. View All» Storage Conditions: As supplied, store at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. After initial defrost, aliquot (for extended storage it is recommended that aliquots be no smaller then 10 μL) and store at -80°C. Snap-freezing in a dry ice/ethanol bath or liquid nitrogen is recommended. As supplied, the enzyme is stable on ice for several hours. View All» Gene Symbol:

• UBE2V2

• EDPF-1

• EDAF-1

• EDPF1

• UEV-2

• UEV2

• DDVit-1

• MMS2

• DDVIT1
  View All» Product Name: UbcH13/Mms2 Conjugating Enzyme View All» UniProt Number: Q15819 View All» Entrez Gene Number: NM_003350.2 View All» Purity: >95% by SDS-PAGE View All» Purification Method: Purified using Ni²⁺ chelation chromatography with Ni2+ in limiting concentration. View All»
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