货号：14-863

品牌：Merck Millipore

规格：300Ug

目录价：￥7090.00

市场价格：￥6026.50

会员价格：￥5672.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Raf-1 RBD, GST View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 300 µg View All» Product Overview: GST fusion-protein, corresponding to the human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. with purity > 50% at full length molecular weight 42 kDa. Specifically binds to and precipitates Ras-GTP from cell lysate. Can be bound to glutathione coated plates prior to precipitating Ras-GTP. View All» Key Applications: Affinity Binding Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text] View All» UniProt Summary: FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.COFACTOR: Binds 2 zinc ions per subunit.SIZE: 648 amino acids; 73052 Da SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain. View All» Species: Human View All» Molecular Weight: 42 kDa View All» Brand Family: Upstate View All» Source: Expressed in E. coli View All» Presentation: 300 μg of Raf-1 RBD, GST in 150 μL in PBS, pH 7.4, 0.5mM PMSF, and 20% glycerol. View All» Storage Conditions: 1 year at -20°C from date of shipment. View All» Gene Symbol:

• RAF1

• cRaf

• Raf-1

• c-Raf

• RAF

• CRAF

• C-RAF
  View All» Product Name: Raf-1 RBD, GST View All» Quality Assurance: Affinity Precipitation of Activated Ras: Raf-1-RBD, GST can be captured on a glutathione coated plate and used to capture GTP activated Ras from HeLa cell lysate. Used 2 μg of Raf-1-RBD, GST per well to capture 25-100 μg of EGF stimulated HeLa cell lysate. Upstate also offers a Ras GTPase ELISA kit (17-424). Note: Due to spontaneous deactivation of Ras, use lysates that have been stored at -70°C for less than 6 month. View All» UniProt Number: P04049 View All» Specific Activity: >50% purity Specifically binds to and precipitates Ras-GTP from cell lysate. Can be bound to glutathione coated plates prior to precipitating Ras-GTP. View All» Entrez Gene Number: NM_002880.2 View All» Purification Method: Glutathione-agarose View All»
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