货号：14-872

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Ube1, recombinant View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 10 µg View All» Product Overview: Recombinant fusion protein containing human Ube1 with an N-terminal His-tag, expressed in sf9 insect cells. View All» Key Applications: Activity Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. View All» UniProt Summary: FUNCTION: SwissProt: P22314 # Activates ubiquitin by first adenylating with ATP its carboxy-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.SIZE: 1058 amino acids; 117849 Da SUBUNIT: Monomer (By similarity).SIMILARITY: SwissProt: P22314 ## Belongs to the ubiquitin-activating E1 family. MISCELLANEOUS: There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. View All» Species: Human View All» Molecular Weight: 121 kDa View All» Brand Family: Upstate View All» Source: sf9 insect cells View All» Presentation: 10 µg recombinant Ube1 in 20 µL of 50mM Tris-HCl, pH 7.5, 270mM Sucrose, 150mM NaCl, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.02% Brij-35, 0.2mM PMSF, and 1mM Benzamidine. Frozen solution. View All» Storage Conditions: Stable for 2 years at -70°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» Gene Symbol:

• UBE1

• MGC4781

• A1S9T

• A1S9

• A1ST

• UBE1X

• GXP1
  View All» Product Name: Ube1, recombinant View All» Alternate Names: ubiquitin-activating enzyme E1 View All» Quality Assurance: Routinely evaluated by ubiquitin assay View All» UniProt Number: P22314 View All» Entrez Gene Number:
  • NM_003334.2

  • NM_153280.1
    View All» Purity: 75% by SDS PAGE and Coomassie blue staining. View All» Purification Method: ProBond Resin chromatography View All»
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