货号：14-925M

品牌：Merck Millipore

规格：

目录价：￥35983.00

市场价格：￥30585.55

会员价格：￥28786.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Wee1, active View All» Qty/Pk: 250 µg View All» Product Overview: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. View All» Key Applications: Kinase Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Species: Human View All» Molecular Weight: 77 kDa View All» Brand Family: Upstate View All» Source: N-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1 amino acids 214-end expressed by baculovirus in Sf21 insect cells. View All» Protein Target: WEE1 View All» Presentation: 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. View All» Storage Conditions: 6 months at -70°C View All» Target Sub-Family: CK1 View All» Promotional Text: This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service. View All» Gene Symbol: WEE1 View All» Product Name: Wee1, active View All» Quality Assurance: Routinely evaluated by phosphorylation of peptide LSNLYHQGKFLQTFCGSPLYRRR. View All» UniProt Number: P30291 View All» Specific Activity: For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. View All» Packaging: Also available in 10μg size as catalog # 14-925 and in bulk as catalog # 14-925-K. View All» Entrez Gene Number: NM_003390 View All» Purification Method: Purified using glutathione agarose. View All»