货号：16-146

品牌：Merck Millipore

规格：

目录价：￥4621.00

市场价格：￥3927.85

会员价格：￥3696.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-IRS1 Antibody, agarose conjugate | 16-146 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: Anti-IRS-1 (06-248) covalently coupled to protein A agarose using dimethylpimelimidate. View All» Specificity: Recognizes IRS1, Mr 160-185kDa. View All» Molecular Weight: 165 kDa View All» Epitope: C-terminal of IRS1 View All» Immunogen: Carboxy-terminal 14 amino acid peptide of rat liver IRS1 (C-YASINFQKQPEDRQ). View All» Isotype: IgG View All» Background Information: Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes. View All» Species Reactivity: All View All» Species Reactivity Note: All View All» Application Notes: Affinity Purification: The antibody-agarose may be useful for affinity purification of proteins that bind IRS1.Suitable for immunoprecipitation and affinity purification. Elution Buffer (IRS1 Released) SDS-Page sample buffer (100%) 0.5M Glycine, pH 2.0 (0%) 0.1M TEA, pH 11.5 (0%) View All» Control: 3T3/A31 mouse fibroblasts cell lysate. View All» Quality Assurance: Routinely evaluated by immunoprecipitating IRS-1 from a RIPA lysate of 3T3/A31 mouse fibroblasts.Immunoprecipitation: 10-25 μg (20-50 μL of 50% slurry) of this lot was used to immunoprecipitate IRS1 from 500 μg of a RIPA lysate of 3T3/A31 mouse fibroblasts. After boiling in SDS-PAGE sample buffer, a small amount of the non-covalently bound IgG light chain (28 kDa) and heavy chain (55 kDa) may be observed. View All» Presentation: Gel immobilized rabbit polyclonal IgG bound to beads; provided as a 50% gel slurry in PBS containing 0.05% azide. Liquid suspension. View All» Storage Conditions: Stable for 6 months at 2-8ºC from date of receipt. View All» UniProt Number: P35568 View All» Entrez Gene Number: NM_005544.1 View All» Gene Symbol:

• HIRS-1

• IRS-1
  View All» Alternate Names: insulin receptor substrate 1 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunoprecipitation View All» UniProt Summary: FUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).SIZE: 1242 amino acids; 131591 Da SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. View All» Brand Family: Upstate View All» Product Name: Anti-IRS1 Antibody, agarose conjugate | 16-146 View All» Concentration: 200 μg of anti-IRS1 bound to 200 μL of beads for a final volume of 400 μL. View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Gel Immobilized View All» Host: Rabbit View All»
  © 金山科研平台是专业的授权总代理区域代理经销平台。
  © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
  © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
  © 本文地址：https://www.16ao.com/thread-147148.htm
  © Merck Millipore代理16-146 ANTI-IRS-1, AGAROSE CONJ. 200UG，产品报价联系微信jinshanbio