货号：16-199

品牌：Merck Millipore

规格：1mg

目录价：￥17784.00

市场价格：￥15116.40

会员价格：￥14227.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes tyrosine-phosphorylated proteins from all species. View All» Molecular Weight: Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected. View All» Epitope: N/A View All» Modifications: Phosphorylation View All» Clone: 4G10® View All» Background Information: Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments. View All» Species Reactivity: Human View All» Application Notes: This product is useful for immunoprecipitation and immunoaffinity chromatography methods. View All» Control: Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells. View All» Quality Assurance: Routinely evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.Immunoprecipitation Analysis: 10 μL of this lot immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells. View All» Purification Method: Protein A purfied View All» Presentation: Gel Immobilized mouse monoclonal antibody covalently coupled to protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.01% Kathon, as a preservative for a total volume of 2 mL. Liquid suspension. View All» Storage Conditions: Stable for 6 months at 2-8ºC from date of receipt. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunoprecipitation View All» Brand Family: Upstate View All» Product Name: Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate View All» Concentration: 1 mg antibody covalently coupled to 1 mL protein A agarose beads for a total volume of 2 mL. View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 1 mg View All» Format: Gel Immobilized View All» Host: Mouse View All»