货号：16-282

品牌：Merck Millipore

规格：200UL

目录价：￥6601.00

市场价格：￥5610.85

会员价格：￥5280.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate | 16-282 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes tyrosine-phosphorylated proteins from all species View All» Molecular Weight: Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected. View All» Immunogen: Phosphotyramine-KLH View All» Isotype: IgG2bκ View All» Background Information: Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments. View All» Species Reactivity: All View All» Species Reactivity Note: All View All» Control: NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes) View All» Quality Assurance: 100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate (Lane 2). View All» Purification Method: Protein G-Sepharose Chromatography View All» Presentation: 20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs. View All» Storage Conditions: Stable for 1 year at 2-8ºC from date of receipt.Note: DO NOT FREEZE View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunoprecipitation View All» Product Name: Anti-Phosphotyrosine, clone 4G10®, Magnetic Bead Conjugate View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 μL View All» Format: Purified View All» Host: Mouse View All»