Merck Millipore代理17-10006 Nitrotyrosine ELISA Kit-试剂-金山科研平台

Merck Millipore代理17-10006 Nitrotyrosine ELISA Kit

2025-06-27

货号：17-10006

品牌：Merck Millipore

规格：

目录价：￥7327.00

市场价格：￥6227.95

会员价格：￥5861.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Nitrotyrosine ELISA View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 1 kit View All» Product Overview: Oxidative stress has been implicated in the pathogenesis of neurodegenerative disorders, cancer, and aging. Oxidative stress occurs as a response to increased oxidants, decreased anti-oxidants, or failure to repair oxidative damage induced by reactive oxygen species (ROS). ROS are free radicals, reactive molecules containing oxygen, or molecules containing oxygen that generate free radicals. ROS include nitric oxide (NO), superoxide (O₂^-), peroxynitrite (ONOO^-), and hydroxyl radical (OH^-). ROS are cleared from cells by the action of superoxide dismutase (SOD), catalase and peroxidases. NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced. ROS may interact to form agents that modify cellular proteins. Superoxide, produced by NADPH oxidase (phox), reacts with NO to form peroxynitrite. Intracellular peroxynitrite can modify proteins by interacting with and nitrating tyrosine residues to form 3-nitrotyrosine. Tyrosine nitration may increase (e.g. sGC, Src, PI3K, Akt), decrease (e.g. Mn-SOD, Ca⁺⁺-ATPase), or have no discernable effect (e.g. p53, VASP, α-Synuclein) on the activity of a particular protein. Upstate's new Nitrotyrosine Assay Kit, Chemiluminescence Detection (Cat. # 17-10006) is a competitive ELISA for the quantitation of tyrosine nitration. The kit includes all reagents needed for measurement of tyrosine nitration, including a pre-coated 96-well plates, Nitrotyrosine peptide standard, a Nitrotyrosine antibody, LumiGLO® chemiluminescent detection substrate, and wash buffers. The assay has a wide dynamic range and high precision, making this assay a valuable new tool for the study of tyrosine nitration. View All» Background Information: Oxidative stress has been implicated in the pathogenesis of neurodegenerative disorders, cancer and aging. Oxidative stress occurs as a response to increased oxidants, decreased antioxidants, or failure to repair oxidative damage induced by reactive oxygen species (ROS). ROS are free radicals, reactive molecules containing oxygen, or molecules containing oxygen that generate free radicals. ROS include nitric oxide (NO), superoxide (O2-), peroxynitrite (ONOO-), and hydroxyl radical (OH-). ROS are cleared from cells by the action of superoxide dismutase (SOD), catalase and peroxidases. NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced. ROS may interact to form agents that modify cellular proteins. Superoxide, produced by NADPH oxidase (phox), reacts with NO to form peroxynitrite. Intracellular peroxynitrite can modify proteins by interacting with and nitrating tyrosine residues to form 3-nitrotyrosine. Tyrosine nitration may increase (e.g. sGC, Src, PI3K, Akt), decrease (e.g. Mn-SOD, Ca++-ATPase), or have no discernable effect (e.g. p53, VASP, α-Synuclein) on the activity of a particular protein. View All» Key Applications: ELISA View All» Species Reactivity: All View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Brand Family: Upstate View All» Sensitivity: 0.1 μM View All» Storage Conditions: Maintain the unopened kit (17-10006-1) at 2-8ºC until expiration date. Maintain the unopened kit (17-10006-2) at -20ºC until expiration date. Avoid repeated freeze/thaw cycles, aliquot if necessary. View All» Standard Curve Range: 0.1 μM - 1600 μM View All» Product Name: Nitrotyrosine ELISA View All» Materials Required but Not Delivered: 1. Multi-channel or repeating pipettes2. Plate shaker (optional)3. Pipettors and tips capable of accurately measuring 1-1000 µL4. Graduated serological pipettes5. 96-well microtiter plate reader with luminescence readout.6. Graphing software for plotting data or graph paper for manual plotting of data7. Microcentrifuge tubes for standard and sample dilutions8. Mechanical vortex9. 1 liter container10. Distilled or deionized water View All» Analytes Available: Nitrotyrosine View All» Components:

1. Streptavidin Capture Plate: (Part No. CS204971) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.
2. U-Bottom Plate: (Part No. CS205781) One clear non-binding U-bottom plate.
3. TBS, 20X: (Part No. 20-190) One bottle (50 mL) of 50X TBS.
4. 20% Tween® 20: (Part No. 20-246) One bottle (3 mL) of 20% Tween® 20 (v/v).
5. LumiGlo Chemiluminescent Substrate Reagent A: (Part No. 20-212C) One bottle (10 mL) of substrate.
6. LumiGlo Chemiluminescent Substrate Reagent B: (Part No. 20-212D) One bottle (10 mL) of substrate.
7. Plate Covers: Two plate covers.
8. Nitrotyrosine Peptide Standard: (Part No. CS204980). One vial containing (200 µL) of Nitrotyrosine Peptide Standard Stock at 4.8mM.
9. 1000X Biotinylated Nitrotyrosine Peptide: (Part No. CS204990). One vial containing (15 µL) of Biotinylated Nitortyrosine Peptide Stock at 450µM.
10. 2000X Anti-Nitrotyrosine Detection Antibody: (Part No. CS204982) One vial (10 µL) of 2000x anti-Nitrotyrosine detection antibody.
11. Gt X Rb, HRP Secondary Antibody: (Part No. 90276) One vial (30 µL) of 2000X goat anti-rabbit HRP conjugate.
12. 30% BSA: (Part No. CS205797) One vial containing 1mL of 30% BSA.
View All» Detection Methods: Chemiluminescence View All»

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