货号：17-10034

品牌：Merck Millipore

规格：25assays

目录价：￥6227.00

市场价格：￥5292.95

会员价格：￥4981.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+ EED View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Trade Name: Upstate (Millipore) View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.The ChIPAb+ EED, set includes the polycomb protein EED antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 138 bp region upstream of human HoxA2 gene. The EED and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of EED associated chromatin. View All» Specificity: Recognizes EED, Mr 62-70 kDa. View All» Molecular Weight: ~62-70 kDa View All» Epitope: a.a. 429-441 View All» Immunogen: A synthetic peptide corresponding to a.a. 429-441 of human EED, conjugated to KLH. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Opossum

• Chicken

• Canine

• Bovine
  View All» Species Reactivity Note: Human, Mouse. Predicted to react with rat, opossum, chicken, canine (dog), and bovine (cow) based on sequence homology. View All» Application Notes: Chromatin Immunoprecipitation:Representative lot data.Sonicated chromatin prepared from Ntera2 cells (3 X 10⁶ cell equivalents per IP) was subjected to chromatin immunoprecipitation using 1 µg of either a normal rabbit IgG or Anti-EED antibody and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of EED associated DNA fragments was verified by qPCR using GAPDH promoter (negative) and HoxA2 (positive) Primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.Please refer to the EZ-Magna A ChIP™ (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.Western Blot Analysis:Representative lot data.Lysates from Jurkat cell lysate were resolved by electrophoresis, transferred to PVDF and probed with anti-EED. Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection (Please see figures). View All» Control: Includes negative control rabbit IgG antibody and primers specific for human HoxA2 upstream region. View All» Quality Assurance: Chromatin Immunoprecipitation:Sonicated chromatin prepared from Ntera2 cells (3 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal rabbit IgG or Anti-EED antibody and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of EED associated DNA fragments was verified by qPCR using Control Primers (Please see figures). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. View All» Presentation: Anti-EED (Rabbit polyclonal IgG). One vial containing 50 μg of affinity purified antibody in 50 μL of 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl, containing 0.05% azide. Store at -20°C.Normal rabbit IgG. 1 vial containing 125 μg of purified rabbit IgG in 125 μL of storage buffer containing 0.1% sodium azide. Store at -20°C.ChIP Primers, HoxA2 upstream. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human HoxA2. Store at -20°C.FOR: AGG AAA GAT TTT GGT TGG GAA GREV: AAA AAG AGG GAA AGG GAC AGA C View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: O75530 View All» Entrez Gene Number: NP_003788 View All» Gene Symbol:
  • EED

  • WAIT-1
    View All» Alternate Names:
    • EED

    • polycomb protein EED

    • WD protein associating with integrin cytoplasmic tails 1

    • WAIT-1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation
        View All» Entrez Gene Summary: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] View All» UniProt Summary: Function: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. Subunit Structure: Interacts with MLL By similarity. Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. Component of the PRC2/EED-EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC, HDAC2, histone H1 and YY1. May interact with ITGA4, ITGAE and ITGB7. May interact with the MA protein of HIV-1.Subcellular Location: Nucleus. Note: Transiently colocalizes with XIST at inactive X chromosomes. Tissue Specificity: Expressed in brain, colon, heart, kidney, liver, lung, muscle, ovary, peripheral blood leukocytes, pancreas, placenta, prostate, spleen, small intestine, testis, thymus and uterus. Appears to be overexpressed in breast and colon cancer.Developmental Stage: Expression peaks at the G1/S phase boundary. Induction: Expression is induced by E2F1, E2F2 and E2F3. Sequence similarities: Belongs to the WD repeat ESC family.Contains 7 WD repeats. View All» Brand Family: Upstate View All» Product Name: ChIPAb+ EED View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 25 assays View All» Host: Rabbit View All» Packaging: 25 assays per set. Recommended use: ~2 μg antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
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