货号：17-10048

品牌：Merck Millipore

规格：

目录价：￥4960.00

市场价格：￥4216.00

会员价格：￥3968.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+ Histone H2A.Z View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Histone H2A.Z set includes the Histone H2A.Z antibody, a negative control antibody (normal sheep IgG), and qPCR primers which amplify a 166 bp region of human GAPDH. The Histone H2A.Z and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Histone H2A.Z -associated chromatin. View All» Specificity: Recognizes Histone H2A.Z, Mr ~15 kDa. View All» Molecular Weight: ~15 kDa View All» Epitope: C-terminus View All» Immunogen: Linear peptide corresponding the C-terminal amino acids 113-127 of human Histone H2A.Z. View All» Background Information: H2A.Z, also known as H2AFZ, is a variant of histone H2A variant that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control. View All» Species Reactivity: Human View All» Species Reactivity Note: Human. Wide species reactivity is expected.Predicted to cross-react with zebrafish, mouse, chicken, rat, rabbit, and Xenopus based on sequence homology. View All» Application Notes: Chromatin Immunoprecipitation:Sonicated chromatin prepared from HeLa cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µg of either Normal Sheep IgG or 1 µg of Anti-Histone H2A.Z and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of Histone H2A.Z associated DNA fragments was verified by qPCR using Control Primers (Part No. 22-004) for a positive, and GAPDH Coding primers as a negative assay (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.Western Blot Analysis:Representative lot dataHeLa lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Histone H2A.Z (1:1000 dilution).Proteins were visualized using a rabbit anti-sheep secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures). View All» Control: Includes negative control sheep IgG antibody and primers specific for human GAPDH. View All» Quality Assurance: Chromatin Immunoprecipitation:Sonicated chromatin prepared from HeLa cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 µg of either Normal Sheep IgG or 1 µg of Anti-Histone H2A.Z and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of H2A.Z associated DNA fragments was verified by qPCR using Control Primers (Please see figures). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. View All» Purification Method: Affinity Purfied View All» Presentation: Anti-Histone H2A.Z (Sheep polyclonal IgG). One vial containing 25 μg of affinity purified sheep polyclonal IgG in PBS, pH 7.4 containing 1% BSA with 0.02% sodium azide, before the addition of 30% glycerol. Store at -20°C. Normal Sheep IgG. One vial containing 25 µg of protein G purified sheep IgG in 0.02 M potassium phosphate, 0.15 M NaCl, pH 7.2, with 0.01% sodium azide, before the addition of 30% glycerol. Store at -20°C.Control Primers. One vial containing 75 μL of 5 μM of each primer specific for human GAPDH. Store at -20°C.FOR: TAC TAG CGG TTT TAC GGG CGREV: TCG AAC AGG AGG AGC AGA GAG CGA View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P0C0S5 View All» Entrez Gene Number: NP_036544 View All» Gene Symbol:

• H2A.z

• H2A/z

• H2AZ

• MGC117173

• H2AFZ
  View All» Alternate Names:
  • H2A histone family, member Z

  • H2AZ histone
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. Several transcript variants encoding different isoforms, have been identified for this gene. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. Ref.7SUBUNIT STRUCTURE:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP By similarity.SUBCELLULAR LOCATION:Nucleus. PTM: Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis By similarity.Not phosphorylated By similarity.SIMILARITY: Belongs to the histone H2A family.MOLECULAR WEIGHT: is 13413.4 Da from positions 2 - 128. Determined by ESI. Monoisotopic, not modified. Ref.8 Ref.10 Ref.11 View All» Brand Family: Upstate View All» Product Name: ChIPAb+ Histone H2A.Z View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 25 assays View All» Format: Affinity Purified View All» Host: Sheep View All» Packaging: 25 assays per set. Recommended use: ~1 μg of antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
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