货号：17-10051

品牌：Merck Millipore

规格：EA

目录价：￥4450.00

市场价格：￥3782.50

会员价格：￥3560.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+ Acetyl-Histone H3 (Lys14) View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Acetyl-Histone H3 (Lys14) set includes the Acetyl-Histone H3 (Lys14) antibody, a negative control normal rabbit IgG, and qPCR primers which amplify a 89 bp region of human c-myc promoter. The Acetyl-Histone H3 (Lys14) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Acetyl-Histone H3 (Lys14)-associated chromatin. View All» Specificity: Recognizes Histone H3 acetylated on Lys14. View All» Molecular Weight: ~17 kDa View All» Epitope: Acetylated Lys14 View All» Immunogen: Acetyl-specific synthetic peptide corres-ponding to residues around Lys14 of Histone H3. View All» Modifications: Acetylation View All» Isotype: IgG View All» Background Information: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. View All» Species Reactivity:

• Human

• Rat
  View All» Species Reactivity Note: Human and rat. View All» Application Notes: Chromatin Immunoprecipitation: Representative lot data.Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Normal rabbit IgG or 2 µL of Anti-acetyl-Histone H3 (Lys14) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of acetyl-Histone H3 (Lys14) associated DNA fragments was verified by qPCR using ChIP Primers c-myc as a positive locus, and myoD promoter primers as a negative locus. (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.ChIP-seq Analysis: Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat# 17-10460), 2 µL of anti-acetyl-Histone H3 (Lys14) antibody (cat# 17-10051), 20 µL Protein A/G beads, and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq instrument. An excess of eighteen million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files. Western Blot Analysis: Representative lot data.Sodium Butyrate-treated HeLa acid extract was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-Acetyl-Histone H3 (Lys14) (1:1000 dilution). To demonstrate specificity, serum was preincubated with modified Histone peptides:Lane 1: No PeptideLane 2: Histone H3 ControlLane 3: Acetyl-Histone H3 (K9, K14)Lane 4: Acetyl Histone H3 (K9)Lane 5: Acetyl-Histone H3 (K14)Lane 6: Penta Acetyl Lys/ArgLane 7: Acetyl-Histone H1 (K26)Lane 8: Acetyl-Histone H1.4 (K26)Lane 9: Histone H1.4 (acetyl K26/phospho K27)Lane 10: Trimethyl-Histone H3 (K4)Lane 11: Dimethyl-Histone H3 (K4)Lane 12: Monomethyl-Histone H3 (K4)Proteins were visualized using a donkey anti-rabbit IgG conjugated to HRP and visualized using a chemiluminescence detection system.Arrow indicates Histone H3 (~17 kDa) (Please see figures).Immunfluorescent IC Analysis:Representative lot data.A previous lot was used in immunofluorescent staining of HeLa cells using Anti-Acetyl-Histone H3 (Lys14) (Please see figures).Immunohistochemistry (Paraffin) Analysis:Representative lot data.A previous lot was used in immunohistochemical staining of paraffin-embedded human adenocarcinoma of uterus using Anti-Acetyl-Histone H3 (Lys14) (Please see figures). View All» Control: Includes negative control normal rabbit IgG and primers specific for human c-myc promoter. View All» Quality Assurance: Chromatin Immunoprecipitation:Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Normal Rabbit IgG or 2 µL of Anti-acetyl-Histone H3 (Lys14) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of acetyl-Histone H3 (Lys14) associated DNA fragments was verified by qPCR using ChIP Primers, c-myc (Please see figures). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. View All» Purification Method: Unpurified View All» Presentation: Anti-Acetyl-Histone H3 (Lys14) (rabbit monoclonal). One vial containing 50 µL of unpurified monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, with 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Store at -20°C.Nornal Rabbit IgG. One vial containing 75 μL of normal rabbit IgG. Store at -20°C.ChIP Primers, c-myc promoter. One vial containing 75 μL of 5 μM of each primer specific for human c-myc promoter. Store at -20°C. FOR: CCC AAA AAA AGG CAC GGA AREV: TAT TGG AAA TGC GGT CAT GC View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P84243 View All» Entrez Gene Number: NP_003484.1 View All» Gene Symbol:
  • H3.3A

  • H3.3B

  • H3F3

  • MGC87782

  • MGC87783

  • OTTHUMP00000035618

  • OTTHUMP00000035619

  • OTTHUMP00000035621
    View All» Alternate Names:
    • H3K14Ac

    • Histone H3 (acetyl K14)

    • H3 histone, family 3A
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Chromatin Immunoprecipitation (ChIP)

      • ChIP-seq

      • Western Blotting

      • Immunocytochemistry

      • Immunoprecipitation

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. View All» UniProt Summary: FUNCTION: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. SUBCELLULAR LOCATION: Nucleus. DEVELOPMENTAL STAGE: Expressed throughout the cell cycle independently of DNA synthesis.PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8sme2). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8sme2) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me), which are linked to gene repression, are underrepresented. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, probably DAPK3. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation on Ser-32 is specific to regions bordering centromeres in metaphase chromosomes.Ubiquitinated By similarity.SEQUENCE SIMILARITIES: Belongs to the histone H3 family.SEQUENCE CAUTION: The sequence CAH73371.1 differs from that shown. Reason: Erroneous gene model prediction. View All» Brand Family: Upstate View All» Product Name: ChIPAb+ Acetyl-Histone H3 (Lys14) View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 25 assays View All» Format: Unpurified View All» Host: Rabbit View All» Packaging: 25 assays per set. Recommended use: ~2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
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