货号：17-10091

品牌：Merck Millipore

规格：

目录价：￥5554.00

市场价格：￥4720.90

会员价格：￥4443.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: CpGenome™ 5-hmC Quantitation Kit View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 96-well plate View All» Product Overview: Ideal for global and tissue-specific detection and quantitation of 5-hmC in genomic DNA, as well as high-throughput screening of activators and inhibitors of 5-hmC modification.Features and Benefits

• Compatible with genomic DNA from cultured cells or tissues

• Includes set of 5-hmC of standards for precise quantitation

• Streamlined, simple, automation-friendly ELISA-based protocol

• Sensitive colorimetric detection with a limit of quantitation down to 0.03% of input DNA.
  View All» Background Information: Rapidly quantify levels of 5-hmC DNA in a wide range of DNA samples with a limit of detection down to 0.03% of input DNA. The CpGenome™ 5-hmC Quantitation Kit enables determination of relative and absolute levels of 5-hydroxymethyl cytosine (5-hmC) in genomic DNA samples. This assay utilizes a rapid, streamlined, automation-compatible, ELISA-based protocol for sensitive quantification of 5-hmC DNA obtained from cultured cells and tissues. With quantitation based on a set of precisely calibrated standards, this assay is ideal for experiments where global and/or tissue-specific detection and quantitation of 5-hmC DNA is required. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Components: Plate Coating Buffer (15 mL)10X Assay Buffer (30 mL)HRP Detection Reagent (15 mL)96-well Assay Plate and 8-well Strip Caps (1 plate/12 strips)5-hmC Antibody (1mg/mL)100x HRP Detection Antibody (1 mg/mL)5-hmC DNA Quantitation Standards - 5 vials for creation of standard curve (100 ng/µL each) View All» Presentation: Two boxes containing all necessary reagents to perform quantitation of 5-hmC levels in a 96-well plate. View All» Storage Conditions: Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 month year from date of shipment when stored and handled as directed. View All» Promotional Text: 5-hmC and 5-mC DNA StandardsGenomic DNA isolated from different tissues with precisely quantitated amounts of 5-mC and 5-hmC. ●CpGenome™ Mouse 5-mC and 5-hmC DNA Standards ●CpGenome™ Human 5-mC and 5-hmC DNA Standards View All» Packaging: Shipped in two separate boxes: Part No. 17-10091-1 containing 2°C to 8°C and room temperature storage components; Part No. 17-10091-2 containing -20°C storage components. View All» Modifications: Hydroxylation View All» Product Name: CpGenome™ 5-hmC Quantitation Kit View All» Materials Required but Not Delivered:
  • Incubator, thermal cyclers

  • Plate reader capable of 405 nm detection

  • Multichannel pipettor (recommended)

  • Parafilm®, Pipette tips, dilution tubes, and other assorted consumables
    View All»
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