货号：17-10256

品牌：Merck Millipore

规格：

目录价：￥4858.00

市场价格：￥4129.30

会员价格：￥3886.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+™ SOX-2 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ SOX-2 set includes the SOX-2 antibody, a Normal mouse IgG, and control primers which amplify a 95 bp region of ChIP Primers, nanog promoter. The SOX-2 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of SOX-2 -associated chromatin. View All» Molecular Weight: ~34 kDa observed View All» Immunogen: Recombinant protein corresponding to human SOX-2. View All» Isotype: IgG2bκ View All» Background Information: SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution. Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain a pluripotent potential. It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS. View All» Species Reactivity:

• Mouse

• Human
  View All» Species Reactivity Note: Demonstrated to react with Mouse and Human. View All» Application Notes: Chromatin Immunoprecipitation: Representative lot data.Sonicated chromatin prepared from NCCIT cells (~2 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 3 µg of either Normal mouse IgG or 3 µg of Anti-SOX-2 and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of SOX-2 associated DNA fragments was verified by qPCR using ChIP Primers, nanog promoter as a positive locus, and MyoD promoter primers as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.Western Blot Analysis: Representative lot data.Mouse embryonic stem cell lysate was probed with Anti-SOX-2 (0.5 μg/mL). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.Arrow indicates SOX-2 (~34 kDa). (Figure 3).Immunocytochemistry Analysis:Representative lot data.RenCX cells were stained with 10 μg/mL of Cat. No. MAB4423, Anti-SOX-2. Reactivity was detected using a Goat Anti-Mouse secondary antibody in Hoescht stain. Positive staining was observed in RenCX cells. View All» Control: Includes normal mouse IgG and primers specific for human nanog. View All» Quality Assurance: Chromatin Immunoprecipitation:Representative lot data.Sonicated chromatin prepared from NCCIT cells (~2 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 3 µg of either Normal Mouse IgG, or 3 µg of Anti-SOX-2 and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of SOX-2 associated DNA fragments was verified by qPCR using ChIP Primers, nanog promoter (Figure 1). Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. View All» Purification Method: Protein G Purified View All» Presentation: Anti-SOX-2 (mouse monoclonal). One vial containing 75 µg of protein G purified monoclonal in buffer containing 0.1 M Tris-glycine (pH7.4), 150mM NaCl, pH 7.4, 0.05% sodium azide before the addition of glycerol to 30%. Store at -20°C.Concentration: 0.7 mg/mLNormal Mouse IgG. One vial containing 125 µg purified mouse IgG in 125 µL storage buffer containing 0.1% sodium azide. Store at -20°C.ChIP Primers, nanog promoter. One vial containing 75µL of 5µM of each primer specific for the promoter region of human nanog. Store at -20°C.FOR: GTT CTG TTG CTC GGT TTT CTREV: TCC CGT CTA CCA GTC TCA CC View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P48431 View All» Entrez Gene Number: NP_003097 View All» Gene Symbol: SOX2 View All» Alternate Names:
  • ANOP3

  • MCOPS3

  • MGC2413
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry
      View All» Entrez Gene Summary: This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). View All» UniProt Summary: FUNCTION: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 By similarity. Critical for early embryogenesis and for embryonic stem cell pluripotency.SUBUNIT STRUCTURE: Interacts with ZSCAN10.SUBCELLULAR LOCATON: Nucleus. View All» Product Name: ChIPAb+™ SOX-2 View All» Concentration: 0.7 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 25 assays View All» Format: Purified View All» Host: Mouse View All» Packaging: 25 assays per set. Recommended use: ~3 μg of antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
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