货号：17-141

品牌：Merck Millipore

规格：

目录价：￥7090.00

市场价格：￥6026.50

会员价格：￥5672.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: TUNEL Apoptosis Detection Kit View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 1 kit View All» Product Overview: For the detection of the endonucleolytic cleavage of chromatin, characteristic of apoptosis. View All» Background Information: Most animal cells can self-destruct via an intrinsic program of cell death that may be triggered by extracellular or intracellular signals. Programmed cell death plays an important role in many biological processes such as morphogenesis and negative selection in the immune system. In addition, dysregulation of programmed cell death may contribute to diseases, for example, cancer, neurodegenerative diseases, and AIDS. Apoptosis is the term for programmed cell death characterized by specific morphologic and biochemical properties. Apoptotic cells typically undergo a series of structural changes: blebbing of the plasma membrane; condensation of the cytoplasm and nuclei; and cellular fragmentation into apoptotic bodies, membrane-bound structures containing condensed cytoplasm, some intact organelles, and nuclear fragments. The most common biochemical property of apoptosis is the endonucleolytic cleavage of chromatin, initially to large fragments of 50-300 kilobase pairs and subsequently to monomers and multimers of 180-200 base pairs. View All» Key Applications: Immunofluorescence View All» Species Reactivity: Mammals View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Brand Family: Upstate View All» Sales Restrictions: This product is not available for sale in Japan. View All» Storage Conditions: Stable for 1 year at -20°C from date of shipment. Once thawed, Blocking solution (Catalog #17-141f) and TB Buffer (Catalog # 17-141e) should be stored at 4°C. View All» Product Name: TUNEL Apoptosis Detection Kit View All» Materials Required but Not Delivered: Cover slips distilled water xylenemoist chamber ethanol (graded series 100%, 90%, 80%) optional counterstain BSA PBS paraformaldehyde Tween-20 gel mount Reaction Buffer: 100 mM Tris, 25 mM MgCl2, 5 mM CaCl2 View All» Quality Assurance: Routinely evaluated by detecting cleaved DNA in HeLa cells after incubation with bovine DNase I View All» Components:

• Proteinase K (PK), Catalog # 17-141a. One vial containing 400 μg in 400 μL.

• TdT (Terminal deoxynucleotidyl Transferase), Catalog # 17-141b. One vial containing 300 Units in 100 μL.

• TdT Buffer, Catalog # 17-141c. Three vials, each vial containing 2 mL.

• Biotin-dUTP (biotinylated deoxyuridine riphosphate), Catalog # 17-141d. One vial containing 100 μL of 250 μM solution.

• TB Buffer (10X), (termination buffer), Catalog # 17-141e. One bottle containing 50 mL of 10X solution.

• Blocking Solution, Catalog # 17-141f. One vial containing 2 mL.

• Avidin-FITC, Catalog # 17-141g. One vial containing 10 μg in 200 μL.

• Bovine DNase I, Catalog # 17-141h. One vial containing 200 μg in 100 μL.
  View All» Detection Methods: Fluorescent View All» Packaging: Kit capacity: 40 assays View All»
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