货号：17-285

品牌：Merck Millipore

规格：1kit

目录价：￥14205.00

市场价格：￥12074.25

会员价格：￥11364.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: TCF Reporter Plasmid Kit View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 1 kit View All» Product Overview: FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type TCF binding sites.TOPflash: Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild type) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. FOPflash containing mutated TCF binding sites is also available as a negative control. View All» Key Applications: Transfection View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: This gene encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described. View All» UniProt Summary: FUNCTION: SwissProt: P20823 # Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.SIZE: 631 amino acids; 67356 Da SUBUNIT: Binds DNA as a dimer.SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Liver.DISEASE: SwissProt: P20823 # Defects in HNF1A may predispose to hepatic adenomas [MIM:142330]. Hepatic adenomas are benign tumors at risk of malignant transformation. Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. & Defects in HNF1A are the cause of maturity onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY [MIM:606391] is a form of diabetes characterized by an autosomal dominant mode of inheritance, age of onset of 25 years or younger and a primary defect in insulin secretion. The clinical phenotype of MODY3 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications. & Defects in HNF1A are a cause of susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100].SIMILARITY: SwissProt: P20823 ## Belongs to the HNF1 homeobox family. & Contains 1 homeobox DNA-binding domain. View All» Brand Family: Upstate View All» Presentation: FOPflash, Catalog # 21-169, Lot # 30860, 5.5 kb. 5 μg affinity purified DNA eluted and aseptically packaged in 50 μL of 10 mM Tris-HCl, pH 8.0, 1 mM EDTA. Frozen solution.TOPflash, Catalog # 21-170, Lot # 27752, 5.5 kb. 5 μg affinity purified DNA eluted and aseptically packaged in 50 μL of 10 mM Tris-HCl, pH 8.0, 1 mM EDTA. Frozen solution. View All» Storage Conditions: Stable for 2 years at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. View All» Gene Symbol:

• TCF1

• MODY3

• HNF1

• HNF-1A

• TCF-1

• HNF1A

• LFB1

• HNF1a
  View All» Product Name: TCF Reporter Plasmid Kit View All» Quality Assurance: Routinely evaluated by DNA Sequence Analysis to confirm the identity of TCF binding sites. View All» Components:
  • FOPflash (mutant TCF binding sites) (Cat.# 21-169)

  • TOPflash (TCF Reporter Plasmid) (Cat.# 21-170)
    View All» UniProt Number:
    • P20823

    • P15884

    • P15923
      View All» Entrez Gene Number: NM_000545.4 View All»
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