货号：17-313

品牌：Merck Millipore

规格：1 KIT C/25 ENSAIOS

目录价：￥5290.00

市场价格：￥4496.50

会员价格：￥4232.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: PP2A Immunoprecipitation Phosphatase Assay Kit View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 25 assays View All» Key Applications: Phosphatase Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).SIZE: 309 amino acids; 35594 Da SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.SUBCELLULAR LOCATION: Cytoplasm.PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily. View All» Brand Family: Upstate View All» Gene Symbol:

• PPP2CA

• PP2CA

• PP2A-alpha

• PP2Ac

• RP-C
  View All» Product Name: PP2A Immunoprecipitation Phosphatase Assay Kit View All» Analytes Available: PP2A View All» Quality Assurance: Routinely evaluated by immunoprecipitation of purified PP2A (14-111) or PP2A from a cellular lysate. The activity is assessed by dephosphorylation of the phosphopeptide (K-R-pT-I-R-R). View All» Components:
  • Malachite Green Solution A (Cat.# 20-105)

  • Malachite Green Additive (Cat.# 20-104)

  • Phosphate Standard (Cat.# 20-103)

  • Threonine Phosphopeptide (K-R-pT-I-R-R) (Cat.# 12-219)

  • Anti-PP2A, C subunit, clone 1D6 (Cat.# 05-421)

  • pNPP Ser/Thr Assay Buffer (Cat.# 20-179)

  • Protein A Agarose

  • 96-Well Microtiter Plate
    View All» UniProt Number: P67775 View All» Packaging: Kit capacity: 25 assays View All» Entrez Gene Number: NP_002706 View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://www.16ao.com/thread-147733.htm
    © Merck Millipore代理17-313 SER/THR IP-PHOSPHATASE ASSAY KIT1 K，产品报价联系微信jinshanbio