货号：17-459

品牌：Merck Millipore

规格：EA

目录价：￥6458.00

市场价格：￥5489.30

会员价格：￥5166.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: phospho-IRS1 (Ser307 mouse/ Ser312 Human) STAR ELISA Kit View All» Trade Name:

• STAR ELISA

• Upstate (Millipore)
  View All» Qty/Pk: 96 assays View All» Background Information: I. TEST PRINCIPLE The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The IRS1 plate is coated with a specific mouse monoclonal capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing IRS1 antigen to be captured in the plate wells. The plate is then washed to remove any unbound non-specific material. The wells are then incubated with a specific rabbit anti-phospho-IRS1 (Ser312) antibody to detect the captured IRS1 on the plate well that is phosphorylated on Ser312. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard curve. II. IRS1 BACKGROUND IRS (Insulin Receptor Substrate) proteins are the effectors of both Insulin and IGF-initiated signaling. Insulin, in addition to working very similarly to a growth factor in stimulating receptor tyrosine kinases, is essential for maintaining glucose homeostasis and regulating carbohydrate, lipid, and protein metabolism. The signaling events of insulin are mediated via a vast and highly integrated network of molecules that controls several processes. There are 4 IRS proteins, 1-4. It is believed that these proteins serve complementary functions in different tissues. The two prominent players are IRS1 and IRS2 with IRS1 playing important roles in skeletal muscle and IRS2 in the liver. The IRS proteins link the insulin receptor with many signaling pathways including the PI3 Kinase/Akt/mTOR and the MAP Kinase pathways. The PI3K/Akt/mTOR pathway is involved with many metabolic actions of insulin signaling. MAPK is involved in the regulation of some genes and works with PI3 Kinase to control cell growth and differentiation. In addition to the insulin receptor tyrosine kinase activity in type II diabetes patients and mice, studies have shown a greater defect in components of the insulin signaling pathway including Insulin Receptor Substrate (IRS) phosphorylation or the activation of Phosphoinositide 3- Kinase (PI3 Kinase). Insulin stimulates the signaling cascade by binding to the insulin receptor through its extracellularα subunits. This stimulates the tyrosine kinase activity of the β subunits of the receptor. The receptor then autophosphorylates itself and phosphorylates the IRS (Insulin Receptor Substrate) proteins that are very important modulators of insulin signaling. IRS binds to the phosphorylated tyrosines of the β subunit of the insulin receptor. They share PH and PTB domains near their N-termini, and multiple Tyr phosphorylation motifs in their C-terminal regions. Proteins which bind to tyrosine-phosphorylated IRS-proteins include PI3 Kinase p85, GRB2, SHP2, Nck, Crk, and Fyn. IRS1 appears to be principally involved in IGF-signaling and cytoskeletal growth. IRS2 appears to be the critical mediator of Insulin signaling, as genetic ablation results in type II diabetes. IRS3 is expressed primarily in adipocytes and is an unusually potent activator of PI3 Kinase. IRS4 lacks the tyrosine residues which in the other IRS-proteins binds to SHP2. View All» Key Applications: ELISA View All» Species Reactivity:
  • Human

  • Mouse

  • Rat
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» UniProt Summary: FUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).SIZE: 1242 amino acids; 131591 Da SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].SIMILARITY: SwissProt: P35568 ## Contains 1 IRS-type PTB domain. & Contains 1 PH domain. View All» Brand Family: Upstate View All» Sensitivity: Sensitivity: 1.5 Units/mL Range of Detection: 1.6 to 100 Units/mL View All» Storage Conditions: Maintain the unopened kit at 2-8°C until expiration date. Precautions • The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data. • Caustic Material: Stop Solution. Caution: Eye, hand, face, and clothing protection should be worn when handling this material. • Safety Warnings and Precautions: This kit is designed for research use only and not recommended for internal use in humans or animals. All chemicals should be considered potentially hazardous and principles of good laboratory practice should be followed. • The Detection Antibody and ELISA Diluent contain sodium azide. Sodium azide may react with copper and lead plumbing to form highly explosive metal azides. Upon disposal, flush with large amounts of water to prevent azide build-up. Avoid contact with skin. • The Anti-Rabbit IgG HRP Conjugate and HRP Diluent contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly. View All» Modifications: Phosphorylation View All» Gene Symbol:
    • IRS1

    • IRS-1

    • HIRS-1
      View All» Product Name: phospho-IRS1 (Ser312) STAR ELISA kit View All» Alternate Names: Insulin Receptor Substrate View All» Materials Required but Not Delivered: 1. Multi-channel or repeating pipettes 2. Plate shaker (optional) 3. Pipettors and tips capable of accurately measuring 1-1000 µL 4. Graduated serological pipettes 5. 96-well microtiter Plate Reader with 450 nm filter 6. Graphing software for plotting data or graph paper for manual plotting of data 7. Microfuge tubes for standard and sample dilutions 8. Mechanical vortex 9. 1 liter container 10. Distilled or deionized water View All» Analytes Available: IRS1 View All» Quality Assurance: Routinely evaluated by ELISA View All» Components:
      • Capture Plate pre-coated with anti-IRS1 antibody: (Part No. 17-459A) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.

      • Anti-phospho-IRS1 (Ser312) detection antibody: (Part No. 17-459B) One bottle (11 mL) of anti- phospho- IRS1 (Ser312) detection antibody containing sodium azide, ready to use.

      • ELISA Diluent: (Part No. 17-459C) One bottle (25 mL) of ELISA Diluent containing sodium azide, ready to use.

      • 25X ELISA Wash Buffer: (Part No. 17-459D) One bottle (50 mL) of 25X ELISA Wash Buffer.

      • Anti-Rabbit IgG HRP conjugate: (Part No. 17-459E) One vial (125 μL) of 100X anti-rabbit HRP conjugate containing thimerosal.

      • HRP Diluent: (Part No. 17-459F) One bottle (25 mL) of HRP Diluent containing thimerosal.

      • TMB Solution: (Part No. 17-459G) One bottle (25 mL) of stabilized tetramethylbenzidine (TMB), ready to use.

      • Stop Solution: (Part No. 17-459H) One bottle (25 mL) of stop solution, ready to use.

      • Phospho-IRS1 (Ser312) Standard: (Part No. 17-459I) Two vials of IRS1 standard, lyophilized.

      • Plate Covers: Two plate covers.
        View All» UniProt Number: P35568 View All» Detection Methods: Chromogenic View All» Entrez Gene Number: NM_005544.1 View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-147871.htm
        © Merck Millipore代理17-459 STAR phospho-IRS1 (S312) ELISA kit，产品报价联系微信jinshanbio