货号：17-473

品牌：Merck Millipore

规格：EA

目录价：￥6330.00

市场价格：￥5380.50

会员价格：￥5064.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: STAR MEK1 ELISA Kit View All» Trade Name:

• STAR

• Upstate (Millipore)
  View All» Qty/Pk: 96 assays View All» Background Information: The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in denatured cell extracts. The MEK1 plate is coated with a specific mouse monoclonal anti-MEK1 capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing MEK1 antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti-MEK1 antibody to detect the captured MEK1 on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard curve. View All» Key Applications: ELISA View All» Species Reactivity:
  • Human

  • Mouse

  • Rat
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. View All» UniProt Summary: FUNCTION: SwissProt: Q02750 # Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.SIZE: 393 amino acids; 43439 Da SUBUNIT: Interacts with MORG1 (By similarity). Interacts with Yersinia yopJ.PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity. & Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.DISEASE: SwissProt: Q02750 # Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio- cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.SIMILARITY: SwissProt: Q02750 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Sensitivity: Sensitivity: 0.16 ng/mL Range of Detection: 0.16 to 10 ng/mL View All» Storage Conditions: 1 year at 4°C View All» Gene Symbol:
    • MAP2K1

    • MAPKK1

    • PRKMK1

    • MEK1

    • MKK1
      View All» Product Name: STAR MEK1 ELISA Kit View All» Materials Required but Not Delivered: 1. Multi-channel or repeating pipettes 2. Plate shaker (optional) 3. Pipettors & tips capable of accurately measuring 1-1000 µL 4. Graduated serological pipettes 5. 96-well microtiter Plate Reader with 450 nm filter 6. Graphing software for plotting data or graph paper for manual plotting of data 7. Microfuge tubes for standard and sample dilutions 8. Mechanical vortex 9. 1 liter container 10. Distilled or deionized water View All» Analytes Available: MEK1 View All» Quality Assurance: Routinely evaluated by ELISA. View All» Components:
      • 1. Capture Plate pre-coated with anti-MEK1 antibody: (Part No. 17-473A) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.

      • 2. Anti-MEK1 detection antibody: (Part No. 17-473B) One bottle (11 mL) of anti-MEK1 detection antibody containing sodium azide, ready to use.

      • 3. ELISA Diluent: (Part No. 17-473C) One bottle (25 mL) of ELISA Diluent containing sodium azide, ready to use.

      • 4. 25X ELISA Wash Buffer: (Part No. 17-473D) One bottle (50 mL) of 25X ELISA Wash Buffer.

      • 5. Anti-Rabbit IgG HRP conjugate: (Part No. 17-473E) One vial (125 µL) of 100X anti-rabbit HRP conjugate containing thimerosol.

      • 6. HRP Diluent: (Part No. 17-473F) One bottle (25 mL) of HRP Diluent containing thimerosol.

      • 7. TMB Solution: (Part No. 17-473G) One bottle (25 mL) of stabilized tetramethylbenzidine (TMB), ready to use.

      • 8. Stop Solution: (Part No. 17-473H) One bottle (25 mL) of stop solution, ready to use.

      • 9. MEK1 Standard: (Part No. 17-473I) Two vials of MEK1 standard, lyophilized.

      • 10. Sample Incubation Buffer: (Part No. 17-73J) One bottle (10 mL) of a sample incubation (denaturing) buffer, ready to use.

      • 11. Plate Covers: Two plate covers.
        View All» UniProt Number: Q02750 View All» Detection Methods:
        • Chromogenic

        • Chromogenic
          View All» Entrez Gene Number: NM_002755.2 View All»
          © 金山科研平台是专业的授权总代理区域代理经销平台。
          © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
          © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
          © 本文地址：https://www.16ao.com/thread-147898.htm
          © Merck Millipore代理17-473 STAR MEK1 (Total) ELISA kit，产品报价联系微信jinshanbio