货号：17-495

品牌：Merck Millipore

规格：

目录价：￥7798.00

市场价格：￥6628.30

会员价格：￥6238.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: PIP3 Quantification TR-FRET Assay View All» Qty/Pk: 1 plate View All» Product Overview: PI 3-Kinases α, β, γ and δ (h) catalyse the phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the presence of ATP and Mg2+. The PIP3 product is detected by displacement of biotin-PIP3 from an energy transfer complex consisting of Europium labelled anti-GST monoclonal antibody, a GSTtagged pleckstrin homology (PH) domain, biotinylated PIP3 and Streptavidin-Allophycocyanin (APC). Excitation of Europium in the complex results in an energy transfer to the APC and a fluorescent emission at 665 nm. The PIP3 product isolated from cell membranes displaces biotin-PIP3 from the complex resulting in a loss of energy transfer and thus a decrease in signal. View All» Key Applications: Kinase Assay View All» Application Notes: This assay requires lipid extraction from cells. A protocol is included with this assay. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. View All» UniProt Summary: FUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.SIZE: 1068 amino acids; 124284 Da SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain. View All» Gene Symbol:

• p110-alpha

• MGC142161

• PI3K

• MGC142163

• PIK3CA
  View All» Product Name: PIP3 Quantification TR-FRET Assay View All» Analytes Available: PI 3-Kinase View All» Components:
  • Black 96-well plate

  • Detection Buffer

  • DM B

  • DM A

  • Stop B

  • PIP3 Standard

  • Lipid Recovery Buffer

  • 1M DTT
    View All» UniProt Number: P42336 View All» Detection Methods:
    • TR-Fret

    • HTRF
      View All» Packaging: Kit capacity: 96-wells View All» Entrez Gene Number: NM_006218.2 View All»
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