货号：17-620

品牌：Merck Millipore

规格：25assays

目录价：￥6227.00

市场价格：￥5292.95

会员价格：￥4981.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+ RNA Pol II View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ RNA Pol II set includes the RNA Pol II antibody, the negative control antibody (mouse IgG), and qPCR primers flanking the human GAPDH promoter, yielding a 166 bp product. The RNA Pol II and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of RNA Pol II associated chromatin. View All» Specificity: Recognizes RNA Pol II View All» Molecular Weight: 210-220 kDa View All» Immunogen: The RNA Pol II antibody is made against a peptide corresponding to the C-terminal domain of RNA Pol II. View All» Isotype: IgG1 View All» Species Reactivity:

• Human

• Mouse

• Yeast (S. cerevisiae)

• Rat
  View All» Species Reactivity Note: Proven to react with human, and mouse. Other species predicted to cross-react based upon sequence conservation are rat and yeast. View All» Application Notes: Western Blot Analysis:3T3 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-RNA polymerase II (0.1 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures). View All» Control: Included negative control antibody mouse IgG and control primers specific for human GAPDH. View All» Quality Assurance: Routinely evaluated by chromatin immunoprecipitation on HeLa nuclear extract. View All» Purification Method: Protein G purfied View All» Presentation: Anti-RNA Pol II (mouse monoclonal IgG1,purified). One vial containing 25 μg of purified antibody in 25 μL volume. Store at -20°C.Normal Mouse IgG. One vial containing 25 ug of mouse IgG in 25 μL volume. Store at -20°C. Control Primers p21. One vial containing 75 μL of 5 μM of each primer specific for a region of the human GAPDH promoter. Store at -20°C.FOR: TAC TAG CGG TTT TAC GGG CGREV: TCG AAC AGG AGG AGC AGA GAGCGA View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. View All» UniProt Number: P24928 View All» Entrez Gene Number: NP_000928 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Brand Family: Upstate View All» Product Name: ChIPAb+ RNA Pol II View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 25 assays View All» Format: Purified View All» Host: Mouse View All» Packaging: 25 assays per set. ~1 μg per chromatin immunoprecipitation View All»
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  © Merck Millipore代理17-620 ChIPAb+ RNA Pol II - ChIP Validated Antibody and Primer Set;store at -20℃，产品报价联系微信jinshanbio