货号：17-646

品牌：Merck Millipore

规格：25assays

目录价：￥6357.00

市场价格：￥5403.45

会员价格：￥5085.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+ HP1γ View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Trade Name: Upstate (Millipore) View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.The ChIPAb+ HP1γ antibody/primer set includes the HP1g antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplifies a 213 bp region within the coding region of the human GAPDH gene. The HP1γ and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HP1γ associated chromatin. View All» Specificity: Recognizes HP1γ, MW: ~25 kDa. View All» Molecular Weight: ~25 kDa View All» Epitope: a.a. 17-173 View All» Immunogen: HP1γ ascites is made against a GST fusion protein corresponding to amino acids 17-173 of human HP1γ. View All» Isotype: IgG1 View All» Species Reactivity:

• Human

• Mouse

• Vertebrates
  View All» Species Reactivity Note: Human, mouse. Not tested in other species. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected. View All» Application Notes: Chromatin Immunoprecipitation:Representative lot data.Sonicated chromatin prepared from HeLa S3 cells (1 X 10⁶ cell equivalents per IP) was subjected to chromatin immunoprecipitation using 5 µg of either a normal mouse IgG or HP1γ antibody and the Magna ChIP G Kit (Cat. # 17-611). Successful immunoprecipitation of HP1γ associated DNA fragments was verified by qPCR using MYOD promoter (negative) and GAPDH coding (positive) (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. View All» Control: Includes negative control mouse IgG antibody and primers specific for of human GAPDH coding region. View All» Quality Assurance: Chromatin Immunoprecipitation:Sonicated chromatin prepared from HeLa S3 cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 5 µg of either a normal mouse IgG or HP1γ antibody and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of HP1γ-associated DNA fragments was verified by qPCR using Control Primers (Please see figures). Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. View All» Presentation: HP1γ (mouse monoclonal IgG). One vial containing 125 μL of ascites. Store at -20°C.Normal Mouse IgG. One vial containing 125 μg purified mouse IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.ChIP Primers GAPDH Coding Region. One vial containing 75 μL of 5 μM of each primer specific for the coding region of human GAPDH. Store at -20°C.FOR: GGC TCC CAC CTT TCT CAT CCREV: GGC CAT CCA CAG TCT TCT GG View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P59665 View All» Entrez Gene Number: NP_009207 View All» Gene Symbol:
  • DEF1

  • DEFA2

  • HNP-1

  • HP-1

  • HP1

  • MGC138393

  • MRS

  • OTTHUMP00000196017
    View All» Alternate Names:
    • defensin, alpha 1

    • defensin, alpha 1, myeloid-related sequence

    • defensin, alpha 2

    • myeloid-related sequence
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene. View All» UniProt Summary: FUNCTION: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. SUBUNIT STRUCTURE: Dimer. SUBCELLULAR LOCATION: Secreted. SEQUENCE SIMILARITIES: Belongs to the alpha-defensin family. View All» Brand Family: Upstate View All» Product Name: ChIPAb+ HP1γ View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 25 assays View All» Host: Mouse View All» Packaging: 25 assays per set. Recommended use: ~5 μg antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
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      © Merck Millipore代理17-646 ChIPAb+ HP1γ - ChIP Validated Antibody and Primer Set;store at -20℃，产品报价联系微信jinshanbio