货号：17-656

品牌：Merck Millipore

规格：1ea

目录价：￥6357.00

市场价格：￥5403.45

会员价格：￥5085.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+ Sox-2, clone 6F1.2 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Recognizes Sox-2, MW: ~34 kDa. View All» Molecular Weight: ~34 kDa View All» Immunogen: Recombinant GST Fusion protein. View All» Clone: 6F1.2 View All» Isotype: IgG2bκ View All» Background Information: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Sox-2, clone 6F1.2 antibody/primer set includes the Sox-2, clone 6F1.2 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 95 bp region within the promoter of the human nanog gene. The Sox-2, clone 6F1.2 antibody and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Sox-2-associated chromatin.Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain pluripotent potential. It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Human, mouse. Not tested in other species. View All» Application Notes: Please refer to the EZ-Magna G ChIP™ (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details. View All» Control: Includes negative control mouse IgG antibody and primers specific for human nanog promoter region. View All» Quality Assurance: Chromatin Immunoprecipitation:Sonicated chromatin prepared from Ntera 2 cells (3 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either a normal mouse IgG, or Anti-Sox-2, clone 6F1.2 antibody and the Magna ChIP™ G Kit (Cat. No. 17-611). Successful immunoprecipitation of Sox-2-associated DNA fragments was verified by qPCR using ChIP Primers, nanog promoter (Figure 1). Please refer to the EZ-Magna ChIP™ G (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P48431 View All» Entrez Gene Number: NP_003097 View All» Gene Symbol:
  • SOX2

  • ANOP3

  • MGC2413

  • MCOPS3
    View All» Alternate Names:
    • Transcription factor SOX-2

    • SOX2
      View All» Components:
      • Anti-Sox-2, clone 6F1.2 (mouse monoclonal IgG), Part No. CS204373. One vial containing 100 μg of protein A purified antibody in 140 μL of 0.02 M phosphate buffer pH 7.6 with 0.25 M NaCl containing 0.1% sodium azide, and 30% glycerol. Store at -20°C.

      • Normal Mouse IgG, Part No. CS200621.One vial containing 125 μg of purified mouse IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.

      • ChIP Primers, nanog promoter, Part No. CS204374. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human nanog. Store at -20°C.FOR: GTT CTG TTG CTC GGT TTT CTREV: TCC CGT CTA CCA GTC TCA CC
        View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
        • Western Blotting

        • Immunoprecipitation
          View All» Entrez Gene Summary: This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). View All» UniProt Summary: FUNCTION: SwissProt: P48431 # Can form ternary complexes with OCT-1 or OCT-3 but acts as a transcriptional activator of FGF-4 enhancer DNA sequences only in conjunction with OCT-3 (By similarity).SIZE: 317 amino acids; 34310 Da SUBCELLULAR LOCATION: Nucleus.DISEASE: SwissProt: P48431 # Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. MCOPS3 is characterized by the association of microphthalmia or clinical anophthalmia with anomalies such as microcephaly, short stature, hypogonadotropic hypogonadism, esophageal atresia and neurologic manifestations.SIMILARITY: SwissProt: P48431 ## Contains 1 HMG box DNA-binding domain. View All» Product Name: ChIPAb+ Sox-2, clone 6F1.2 View All» Concentration: 0.72 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 25 assays View All» Format: Purified View All» Host: Mouse View All» Packaging: 25 assays per set. Recommended use: ~4 μg antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
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