货号：17-662

品牌：Merck Millipore

规格：25assays

目录价：￥6227.00

市场价格：￥5292.95

会员价格：￥4981.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+ EZH2, clone AC22 View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Trade Name: Upstate (Millipore) View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ EZH2, clone AC22 antibody/primer set includes the EZH2, clone AC22 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 135 bp region within the promoter of the human MYT-1 gene. The EZH2, clone AC22 antibody and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of EZH2-associated chromatin. View All» Specificity: Recognizes EZH2, MW: ~85-100 kDa. View All» Molecular Weight: ~85 kDa View All» Epitope: a.a. 353-451 View All» Immunogen: EZH2, clone AC22 purified antibody is made against a GST fusion protein corresponding to amino acids 353-451 of human EZH2. View All» Isotype: IgG2a View All» Species Reactivity:

• Human

• Mouse

• Vertebrates

• Rat
  View All» Species Reactivity Note: Human, mouse, rat. Not tested in other species. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected. View All» Application Notes: Chromatin Immunoprecipitation:Representative lot data.Sonicated chromatin prepared from HeLa S3 cells (4 X 10⁶ cell equivalents per IP) was subjected to chromatin immunoprecipitation using 1 µg of either a normal mouse IgG or Anti-EZH2, clone AC22 antibody and the Magna ChIP G Kit (Cat. # 17-611). Successful immunoprecipitation of EZH2-associated DNA fragments was verified by qPCR using GAPDH promoter (negative) and MYT-1 promoter (positive) primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.Immunoprecipitation Analysis:Representative lot data.HeLa nuclear cell extract was Immunoprecipitated with either anti-EZH2, clone AC22 or mouse IgG and then resolved by electrophoresis, transferred to PVDF and probed with Cat. # 05-1319 anti-EZH2, clone BD43 (1:1000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP (Cat. # AP124P) and a chemiluminescence detection system.(Please see figures).Lane 1: Hela nuclear extract immunoprecipitate with anti-EZH2, clone AC22.Lane 2: Hela nuclear extract immunoprecipitate with IgG. View All» Control: Includes negative control mouse IgG antibody and primers specific for of human MYT-1 promoter region. View All» Quality Assurance: Chromatin Immunoprecipitation:Sonicated chromatin prepared from HeLa S3 cells (4 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-EZH2, clone AC22 antibody and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of EZH2-associated DNA fragments was verified by qPCR using Control Primers (Please see figures). Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. View All» Presentation: EZH2, clone AC22 (mouse monoclonal IgG2a). One vial containing 50 μg of protein G purified antibody in 50 μL PBS containing 0.05% sodium azide. Store at -20°C.Normal Mouse IgG. Two vials, each containing 25 μg purified mouse IgG in 25 μL storage buffer containing 0.1% sodium azide. Store at -20°C.ChIP Primers MYT-1 promoter Region. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human MYT-1. Store at -20°C.FOR: ACA AAG GCA GAT ACC CAA CGREV: GCA GTT TCA AAA AGC CAT CC View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: Q15910 View All» Entrez Gene Number: NP_004447.2 View All» Gene Symbol:
  • EZH2

  • KMT6
    View All» Alternate Names:
    • Enhancer of zeste homolog 2

    • Lysine N-methyltransferase 6

    • enhancer of zeste 2

    • enhancer of zeste homolog 2

    • ENX-1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunoprecipitation

      • ChIP-seq
        View All» Entrez Gene Summary: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. View All» UniProt Summary: FUNCTION: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.SUBUNIT STRUCTURE: Binds ATRX via the SET domain Probable. Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2.SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. DEVELOPMENTAL STAGE: Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.INDUCTION: Expression is induced by E2F1, E2F2 and E2F3. Expression is reduced in cells subject to numerous types of stress including UV-, IR- and bleomycin-induced DNA damage and by activation of TP53/p53. PTM: Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. SEQUENCE SIMILARITIES: Belongs to the histone-lysine methyltransferase family. EZ subfamily.Contains 1 SET domain.CAUTION: Two variants of the PRC2 complex have been described, termed PRC3 and PRC4. Each of the three complexes may include a different complement of EED isoforms, although the precise sequences of the isoforms in each complex have not been determined. The PRC2 and PRC4 complexes may also methylate 'Lys-26' of histone H1 in addition to 'Lys-27' of histone H3 (Ref.15 and Ref.19), although other studies have demonstrated no methylation of 'Lys-26' of histone H1 by PRC2 View All» Brand Family: Upstate View All» Product Name: ChIPAb+ EZH2, clone AC22 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 25 assays View All» Host: Mouse View All» Packaging: 25 assays per set. Recommended use: ~2 μg antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
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