货号：17-720

品牌：Merck Millipore

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目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: H2A.X (Ser139) Dual Detect CELISA ASSAY Kit (Fluorogenic Detection) View All» Trade Name: Dual Detect CELISA View All» Qty/Pk: 96 Assays View All» Product Overview: Dual-Detect CELISA is a simple and efficient cell-based ELISA assay to measure the relative expression levels of both the total and the phosphorylated form of H2A.X in whole cells in a 96 well microplate format using dual fluorescent detection. This allows for the normalization of total protein between wells, thus allowing a more accurate measurement of phosphorylation levels among different cells and/or growth conditions. Cells are cultured directly in the microplate and stimulated as desired. They are then fixed and simultaneously incubated with two primary antibodies. One is a phosphospecific antibody directed against the phosphorylated epitope that correlates with H2A.X activation and the other is a total antibody that recognizes the H2A.X target protein, regardless of phosphorylation status. Both the total target protein and the phosphorylated protein are measured in a single well using a double immunoenzymatic labeling procedure, including both horseradish-peroxidase (HRP) and alkaline phosphatase (AP), and two spectrally distinct fluorogenic substrates for HRP and AP. The fluorescence of the phosphorylated protein is normalized to the total protein in each well, thus reducing well-to-well variations. View All» Background Information: The histone H2A.X protein is a variant member of the H2A family of histones that is distinguished from other H2A histones by a unique carboxy-terminal sequence. This unique sequence is highly conserved throughout eukaryotic evolution [reviewed in 1] and is rapidly phosphorylated by ATM or ATR at the fourth residue from the carboxy-terminus (Serine 139 in mammalian H2A.X) in response to DNA double-strand breaks (DSBs) [2]. Phosphorylation of H2A.X is important in the formation of a stable repair complex at the site of DNA damage.H2A.X phosphorylation is a very rapid response to DNA damage, occurring within as little as one minute after exposure to ionizing radiation [2]. Phosphorylation of H2A.X occurs irrespective of the cause of the DNA DSBs and phospho-H2A.X has been observed in response to environmental stresses that result in DSBs as well as programmed cellular events, including DNA rearrangement and apoptosis. View All» Key Applications: ELISA View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3,and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. View All» UniProt Summary: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. View All» Brand Family: Upstate View All» Storage Conditions: Kit components arrive on dry ice, and must immediately be stored at the temperatures specified on the vials.This kit is shipped at -20°C. Upon receipt, check individual components for storage conditions. The cell culture plate should be stored at room temperature. The 20X TBS, 20% Tween® 20, 10% BSA and Substrate Diluent should be stored at 4°C. All other components are stored at -20°C. Aliquot the -20°C components, if necessary, to avoid freeze-thaw cycles.Kit components are stable for 3 months from date of shipment when stored as directed. View All» Modifications: Phosphorylation View All» Gene Symbol:
  • H2A.X

  • H2AFX

  • H2A histone family, member X
    View All» Product Name: H2A.X (Ser139) Dual Detect CELISA ASSAY Kit (Fluorogenic Detection) View All» Alternate Names: H2A.XH2AFXH2A histone family, member X View All» Quality Assurance: Quality Control Testing: The reagents in this kit have been optimized to reduce background signal while maintaining a high signal to noise ratio. Quality control testing is performed using the enclosed kit components while following the protocol described. View All» Components: 500X anti-H2A.X (Total) Antibody500X anti-phospho-H2A.X (Ser139) AntibodyBlack Clear Bottom 96-well Cell Culture Plate20% Tween® 20 (v/v)20X TBS10% BSA in TBS (Blocking Buffer) Substrate Diluent500X Goat anti-Mouse HRP Detection Reagent500X Goat anti-Rabbit AP Detection Reagent 200X HRP Substrate:200X AP Substrate View All» UniProt Number: P16104 View All» Detection Methods: Fluorescent View All» Entrez Gene Number: NP_002096 View All»
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