货号：30-052

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Phosphatase Substrate Set View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 1 kit View All» Product Overview: Millipore's ready-to-screen Upstate Phosphatase Substrate Set is comprised of a total of 360 phosphopeptides derived from human phosphorylation sites. The Phosphatase Substrate Set comes in a 384-well microtiter plate (250 pmol per well) with 10 calibration phosphate standards. Additionally, several empty wells are available for positive and negative controls (wells P12-P24). Subsequent to incubation with the target phosphatase, released phosphate ions can be detected by complexation to phosphate-binding dyes, i.e. molybdate/malachite green. The sensitivity of the assay is sufficient to detect low nanogram levels of phosphatase using the molybdate/malachite green dye (Van Veldhoven, P.P & Mannaerts, G.P. (1987) Anal. Biochem. 161, 45-48; Ekman, P. & Jager, O. (1993) Anal. Biochem. 241, 138-41; Harder, K.W. et al. (1994) Biochem. J. 298, 395-405). Please use the provided control plate to determine the phosphate content of every single component used for the assay buffer. Obviously, any exogenous phosphate ions introduced with the reagents would corrupt the results of your experiment. The data CD-ROM included with the set contains all information needed for the detailed analysis of your data including peptide sequences as well as the Swiss-Prot accession numbers for the proteins containing these phosphosites. In case the individual human peptide sequence is also found in other organisms, all respective Swiss-Prot accession numbers are given. View All» Key Applications: Kinase Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Brand Family: Upstate View All» Storage Conditions: Phosphatase Substrate Set microtiter plates should be stored at -20°C. All other components may be stored at room temperature. View All» Product Name: Phosphatase Substrate Set View All»