货号：30-054

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Phosphatase Peptide Microarray - Tyrosine View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 1 kit View All» Product Overview: Millipore's ready-to-screen Upstate Phosphatase Peptide Microarrays (PhosphoSites-Tyrosine) are comprised of a total of 1536 phosphotyrosine-containing peptides derived from human phosphorylation sites. The 13meric peptides represent the amino acids around the phosphorylation site with the phosphotyrosine residue in the middle position. An optimized hydrophilic linker moiety is inserted between the glass surface and displayed phosphopeptide to avoid false negatives caused by steric hindrance. The peptides are displayed in three identical subarrays resulting in 4 glass slides with 3 x 384 = 1152 immobilized peptides on each slide, with each of the 1536 peptides mounted in triplicate (4 slides, each with a seperate group of 384 peptides in triplicate). Subsequent to incubation with the enzyme, dephosphorylation can be detected after treatment with fluorescently labeled anti-phosphotyrosine antibody by imaging the microarray. Dephosphorylated peptides will result in a decrease of fluorescence signal intensity. The data CD-ROM included with the microarrays contains all information needed for the detailed analysis of your data including peptide sequences as well as Swiss-Prot accession numbers for the proteins containing these cleavage sites. View All» Key Applications: Kinase Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Brand Family: Upstate View All» Storage Conditions: Storage: • Optimal storage conditions for peptide microarray slides are in a cool (approx. 4°C / 39°F) and dry environment. Peptide microarrays are stable for at least 18 month when stored at 4°C (39°F). • Do not freeze the microarray slides for prolonged storage. Handling: • Always handle the delicate microarray slides with care. • Never touch the microarray slide surface. • Always wear laboratory gloves when handling Phosphatase Peptide Microarrays. • Please hold microarray slides at the end, which carries the engraved data label. This label provides for unique identification of the array. • Please take care when dispensing solutions onto the microarray surface. Make sure not to touch the surface with pipette-tips or dispensers. • Never wipe the surface of the slide with a cloth. • Never use other chemicals as described. Inappropriate chemicals may destroy the chemical bonding of the peptides to the glass surface. • Avoid dust or other particles during each step of the experiment. Dust particles and resulting scratches will cause artefacts during the final signal readout. • Please filter all solutions for the washing steps through 2 µm, preferably 0.4 µm particle filters before use. View All» Product Name: Phosphatase Peptide Microarray - Tyrosine View All»