货号:4500-0250
品牌:Merck Millipore
规格:
目录价:¥5144.00
市场价格:¥4372.40
会员价格:¥4115.20
金山科研平台,产品价格货期咨询微信:jinshanbio
Description:
Guava Mitochondrial Depolarization Kit
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Trade Name:
Guava
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Qty/Pk:
100 Tests
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Product Overview:
Guava Mitochondrial Depolarization Assay for detection of early apoptosisThere is growing interest within the scientific community to evaluate changes in the mitochondrial membrane potential and further define its role in initiating apoptosis, cell cycle, and other cellular processes. Automated single-cell analysis is the technique of choice due to its sensitivity and high reproducibility. Microplate protocolGuava Technologies has optimized an assay format for 96-well plates that combines the sensitivity of a single-cell based method with the throughput and ease-of-use of a microplate reader. The Guava® Mitochondrial Depolarization Assay for the Guava EasyCyte™ Plus System allows you to monitor the loss of mitochondrial membrane potential through the use of the fluorescent cationic dye, JC-1. An optional dead cell dye, 7-AAD, is also included for discerning apoptotic cells from dead cells. Distinguishes healthy, early apoptotic, and dead cellsIn healthy, non-apoptotic cells, the JC-1 dye enters the mitochondrial matrix, accumulates as aggregates and stains the mitochondria bright red. In apoptotic cells, the mitochondrial membrane potential collapses, and the JC-1 enters the cytoplasm as a monomeric form where it fluoresces green. Results are automatically calculated and presented in a statistical format that is easy to interpret, with total cell counts and percentages for those cell populations that are either healthy, apoptotic, or dead. For use with EasyCyte Mini, EasyCyte Plus. Click here for more information regarding Instrument Compatibility.
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Components:
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JC-1
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7-AAD
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Detailed Protocol
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Product Name:
Guava Mitochondrial Depolarization Kit
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Flow Cytometry Software Type:
Assay Specific Module
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Flow Cytometry Market:
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Bioprocess
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Research
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Flow Cytometry Research Subcategory:
Early
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