货号：46-712

品牌：Merck Millipore

规格：EA

目录价：￥3253.00

市场价格：￥2765.05

会员价格：￥2602.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: MILLIPLEX MAP Phospho Lck (Tyr394) MAPmate, 46-712 View All» Trade Name: MILLIPLEX View All» Qty/Pk: 1 kit View All» Product Overview: The MILLIPLEX MAP Phospho Lck (Tyr394) MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detect the presence of phosphorylated Lck (Tyr394) in cell lysates using the Luminex® 100™ IS, 200™, or HTS system. Each MAPmates pair is ordered individually and may be combined for simultaneous multiplex analysis of cellular events. The MILLIPLEX MAP Cell Signaling Buffer and Detection Kit is ordered separately and consists of a common set of reagents needed for performing MAPmates assays. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEX MAP HeLa Cell Lysate: Unstimulated included in the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit may be utilized as a negative control for this target, while the Ramos Cell Lysate: Pervanadate included with this MAPmates pair may be utilized as a positive control. View All» Background Information: Lymphocyte-specific protein-tyrosine kinase (Lck):• Bound to the cytoplasmic domain of either CD4 or CD8• Functions as a key signaling protein in the TCR (T-cell receptor) complex• Rearrangement of Lck may play a role in the lymphocyte malignancy View All» Key Applications: Multiplexing View All» Application Notes: • This assay requires 25 μL diluted cell lysate (recommended starting concentration is 20 μg cell lysate/well)• This is an overnight (4°C) or two-hour (RT) incubation assay; however, for best results an overnight incubation is recommended.• MAPmate is compatible with both Assay Buffer 1(Catalog # 43-010) and Assay Buffer 2 (Catalog # 43-041) View All» Species Reactivity: Human View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Storage Conditions: Recommended storage for kit components is 2 - 8°C View All» Modifications: Phosphorylation View All» Gene Symbol:

• LCK

• p56-LCK

• p56lck

• YT16

• pp58lck

• LSK
  View All» Product Name: MILLIPLEX MAP Phospho Lck (Tyr394) MAPmate, 46-712 View All» Materials Required but Not Delivered: • MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602)• Cell lysates or cell extracts harboring protein(s) of interest prepared using the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602)• Protease inhibitors (Millipore Catalog #20-201 or equivalent)• Luminex Sheath Fluid (Luminex Catalog #40-50000) View All» Analytes Available: Lck (Tyr394) View All» Configuration: Single Plex View All» Components:
  • MILLIPLEX MAP Anti-Lck Beads (20X)

  • MILLIPLEX MAP Anti-phospho Lck (Tyr394), biotin (20X

  • MILLIPLEX MAP Ramos Cell Lysate: Pervanadate
    View All» UniProt Number: P06239 View All» Panel Type: Cell Signaling View All» Detection Methods: Luminex xMAP View All» Entrez Gene Number: NP_001036236 View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://www.16ao.com/thread-150423.htm
    © Merck Millipore代理46-712 Phospho Lck (Tyr394) MAPmates™，产品报价联系微信jinshanbio