货号：70-500

品牌：Merck Millipore

规格：

目录价：￥5563.00

市场价格：￥4728.55

会员价格：￥4450.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: EZ-TFA (Universal Transcription Factor Assay, colorimetric) View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 96 assays View All» Background Information: The UPSTATE Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts. This assay combines the principle of the electrophoretic mobility shift assay (EMSA) with the 96-well based enzyme-linked immunosorbent assay (ELISA), enabling manual or high-throughput sample analysis with greater sensitivity than conventional EMSA assays. After certain parameters are optimized, the entire assay takes less than 4 hours to complete with minimal hands-on time. The versatile set up allows for a flexible assay design and because the binding reaction occurs in solution, various parameters can be optimized such as probe titration, competitive oligonucleotide concentration, or treatment conditions. This kit contains the reagents and instructions necessary to develop an assay to monitor the DNA binding activity of any transcription factor of interest, or as a kit component for Upstate developed target specific Transcription Factor Assays. The Universal kit also allows for the design of native promoter capture probe sequences to interrogate specific transcription factor binding. View All» Key Applications:

• ELISA

• Activity Assay
  View All» Species Reactivity:
  • Rabbit

  • Mouse
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Brand Family: Upstate View All» Storage Conditions: Upon receipt, store components at the temperatures indicated on the labels. Various components to be stored at 4°C, -20°C, or -80°C. View All» Product Name: EZ-TFA (Universal Transcription Factor Assay, colorimetric) View All» Materials Required but Not Delivered: Reagents Cells, stimulated or treated as needed for the experimental system Capture probe Specific competitor Primary antibody Nuclear extraction kit (Catalog # 2900) Nuclease free water (Catalog # 3098)Equipment Heating Block Spectrophotometric Plate Reader View All» Quality Assurance: Routinely evaluated by performing the EZ-TFA with a target specific module. View All» Components:
    • Transcription Factor Assay Plate, Catalog # 90268

    • Blocking Reagent, Catalog # 90273

    • 10X Annealing Buffer, Catalog # 2007044

    • Substrate (TMB/E), Catalog # 90348

    • Stop Solution, Catalog # 60193

    • TFA Buffer, 5X, Catalog # 90272

    • Gt X Rb, HRP Secondary Antibody, Catalog # 90276

    • Gt X Ms, HRP Secondary Antibody, Catalog # 2003482
      View All» Host: Goat View All» Detection Methods: Chromogenic View All»
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